CoraLite®555-conjugated Acetyl-Tubulin (Lys40) Monoclonal antibody

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  IF Staining of hTERT-RPE1 using CL555-66200

  Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using CoraLite®555 Acetyl-Tubulin (Lys40) antibody (CL555-66200, Clone: 7E5H8 ) at dilution of 1:200.

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  IF Staining of MDCK using CL555-66200

  Immunofluorescent analysis of (4% PFA) fixed MDCK cells using CoraLite®555 Acetyl-Tubulin (Lys40) antibody (CL555-66200, Clone: 7E5H8 ) at dilution of 1:200.

Acetyl-Tubulin (Lys40) Monoclonal Antibody for IF/ICC

Cat No. CL555-66200

Clone No.7E5H8

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, canine

Applications

IF/ICC

Synonyms

Ac Tubulin (Lys40), Ac Tubulin Lys40, Acetyl Tubulin, Acetyl Tubulin (Lys40), Acetyl Tubulin Lys40

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Tested Applications

• Positive IF/ICC detected in: MDCK cells, hTERT-RPE1 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL555-66200 targets Acetyl-Tubulin (Lys40) in IF/ICC applications and shows reactivity with human, mouse, rat, pig, canine samples.

• Tested Reactivity: human, mouse, rat, pig, canine
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: tubulin, alpha 1a
• Calculated Molecular Weight: 52 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: NM_006009
• Gene Symbol: Alpha Tubulin
• Gene ID (NCBI): 7846
• RRID: AB_2919679
• Conjugate: CoraLite®555 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 557 nm / 570 nm
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q71U36
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

What is the molecular weight of acetyl-α-tubulin?

The molecular weight of acetylated tubulin is 52 kD.

Where does the acetylation of Lys 40 occur?

This acetylation occurs inside the microtubule lumen by the α-tubulin acetyltransferase 1 (αTAT1) (PMID: 29207274).

How can acetylation be reversed?

Acetylation of Lys 40 can be reversed by deacetylase 6 (HDAC6), which is mostly cytoplasmic that also deacetylates Hsp90, and sirtuin 2 (SIRT2), which also mainly cytoplasmic and uses NAD as a coenzyme. Unlike HDAC6, SIRT uses both polymerized and soluble tubulin as substrates. Deacetylases are believed to be more active on soluble tubulin, while acetylases function preferentially on stable polymers (PMIDs: 29207274, 30079247, 19185337).

What is the function of acetylation?

Acetylation is a conserved post-translational modification of alpha tubulin at Lys 40 during tubulin assembly, and it correlates to increased microtubule stability and intracellular transport (PMIDs: 29207274, 30079247, 20940043).

Is acetylation of α-tubulin strictly associated with stable microtubules?

Not necessarily, as acetylation can have other effects on microtubule subpopulations (PMID 20940043).

Is ac-tubulin found only in cilia?

Acetylated-α-tubulin is located in cytoplasmic tubulin as well as in cilia; therefore, it is not strictly region-specific (PMID: 30079247).

What are the cellular effects of tubulin acetylation?

Microtubule acetylation seems to provide a critical role in neuronal development and function, and while its effect on cancer cells remains unclear, it has been shown that decreased acetylated α-tubulin impairs neuronal cell line migration. The post-translational modification may also help regulate organelle-independent signalling throughout the cell, supporting the notion of a microtubule network serving as a coordinator of cellular signaling (PMIDs: 29207274, 25503560, 20940043, 19185337).