CoraLite®555-conjugated acetylated Tubulin(Lys40) Monoclonal antibody

acetylated Tubulin(Lys40) Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, Canine, pig

Applications

IF

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

7E5H8

Cat no : CL555-66200

Synonyms

alpha tubulin, Alpha tubulin 3, B ALPHA 1, FLJ25113, LIS3, TUBA1A, TUBA3, Tubulin alpha 1A chain, Tubulin alpha 3 chain, Tubulin B alpha 1, tubulin, alpha 1a



Tested Applications

Positive IF detected inMDCK cells
For other applications, we recommend the unconjugated version of this antibody, 66200-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL555-66200 targets acetylated Tubulin(Lys40) in IF applications and shows reactivity with human, mouse, rat, Canine, pig samples.

Tested Reactivity human, mouse, rat, Canine, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name tubulin, alpha 1a
Calculated molecular weight 52 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberNM_006009
Gene symbol TUBA1A
Gene ID (NCBI) 7846
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission maxima wavelengths555 nm / 570 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

What is the molecular weight of acetyl-α-tubulin?

The molecular weight of acetylated tubulin is 52 kD.

 

Where does the acetylation of Lys 40 occur?

This acetylation occurs inside the microtubule lumen by the α-tubulin acetyltransferase 1 (αTAT1) (PMID: 29207274).

 

How can acetylation be reversed?

Acetylation of Lys 40 can be reversed by deacetylase 6 (HDAC6), which is mostly cytoplasmic that also deacetylates Hsp90, and sirtuin 2 (SIRT2), which also mainly cytoplasmic and uses NAD as a coenzyme. Unlike HDAC6, SIRT uses both polymerized and soluble tubulin as substrates. Deacetylases are believed to be more active on soluble tubulin, while acetylases function preferentially on stable polymers (PMIDs: 29207274, 30079247, 19185337).

 

What is the function of acetylation?

Acetylation is a conserved post-translational modification of alpha tubulin at Lys 40 during tubulin assembly, and it correlates to increased microtubule stability and intracellular transport (PMIDs: 29207274, 30079247, 20940043).

 

Is acetylation of α-tubulin strictly associated with stable microtubules?

Not necessarily, as acetylation can have other effects on microtubule subpopulations (PMID 20940043).

 

Is ac-tubulin found only in cilia?

Acetylated-α-tubulin is located in cytoplasmic tubulin as well as in cilia; therefore, it is not strictly region-specific (PMID: 30079247).

 

What are the cellular effects of tubulin acetylation?

Microtubule acetylation seems to provide a critical role in neuronal development and function, and while its effect on cancer cells remains unclear, it has been shown that decreased acetylated α-tubulin impairs neuronal cell line migration. The post-translational modification may also help regulate organelle-independent signalling throughout the cell, supporting the notion of a microtubule network serving as a coordinator of cellular signaling (PMIDs: 29207274, 25503560, 20940043, 19185337).


Protocols

Product Specific Protocols
IF protocol for CL555 acetylated Tubulin(Lys40) antibody CL555-66200Download protocol
Standard Protocols
Click here to view our Standard Protocols