CoraLite®594-conjugated Acetyl-Tubulin (Lys40) Monoclonal antibody

Acetyl-Tubulin (Lys40) Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, Canine, pig and More (1)

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

Publications

3

CloneNo.

7E5H8

Cat no : CL594-66200

Synonyms

Ac tubulin, acetylated alpha tubulin, Acetylated tubulin, acetylated Tubulin (Lys40), acetylated Tubulin(Lys40), acetylated-a-tubulin, acetyl-a-tubulin, Ac-tubulin, alpha tubulin, Alpha tubulin 3, B ALPHA 1, LIS3, TUBA1A, TUBA1A(ace-40Lys), TUBA3, tubulin, Tubulin alpha 1A chain, Tubulin alpha 3 chain, Tubulin B alpha 1, tubulin, alpha 1a

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Validation Data Gallery

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  IF Staining of MDCK using CL594-66200

  Immunofluorescent analysis of (4% PFA) fixed MDCK cells using CL594-66200 (Acetyl-Tubulin (Lys40) antibody) at dilution of 1:100.

• 

  IF Staining of MDCK using CL594-66200

  Immunofluorescent analysis of (4% PFA) fixed MDCK cells using CL594-66200 (Acetyl-Tubulin (Lys40) antibody) at dilution of 1:100.

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Tested Applications

• Positive IF/ICC detected in: MDCK cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 3 publications below

Product Information

CL594-66200 targets Acetyl-Tubulin (Lys40) in IF/ICC applications and shows reactivity with human, mouse, rat, Canine, pig samples.

• Tested Reactivity: human, mouse, rat, Canine, pig
• Cited Reactivity: zebrafish
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: tubulin, alpha 1a
• Calculated Molecular Weight: 52 kDa
• GenBank Accession Number: NM_006009
• Gene Symbol: Alpha Tubulin
• Gene ID (NCBI): 7846
• RRID: AB_2883533
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Tubulin, composed of heterodimers of alpha and beta tubulin, is the mainly component of microtubules which play important roles in cell motility, mitosis, and intracellular vesicle transport. Both alpha and beta tubulin undergo several posttranslational modifications such as polyglutamylation and acetylation/deacetylation. Tubulin acetylation occurs on lysine-40 at the N-terminal of alpha tubulin and is conserved across species. The histone deacetylase HDAC6 and SIRT2 has been identified as tubulin deacetylases. Reversible acetylation of alpha tubulin may be implicated in regulating microtubule stability, cell motility, and axon regeneration. The level of acetylated tubulin has been linked to epithelial malignancies and sensitivity to chemotherapy. In addition, acetylated tubulin has been widely used as a marker for primary cilia. This antibody is specific to the acetylated tubulin; it does not recognize non-acetylated tubulin. (24268707, 23881549) And this anitbody is CL594(Ex/Em 593 nm/614 nm) conjugated.

Publications

Species	Application	Title
	IF	J Neurosci; Comparison of ciliary targeting of two rhodopsin-like GPCRs: role of C-terminal localization sequences in relation to cilium type.; Authors - Abhishek Chadha
	IF	Curr Biol; Cones and cone pathways remain functional in advanced retinal degeneration; Authors - Erika M Ellis
zebrafish	IF	Cell Biosci; IK is essentially involved in ciliogenesis as an upstream regulator of oral-facial-digital syndrome ciliopathy gene, ofd1; Authors - Hye In Ka