ZC3H12D Recombinant antibody, PBS Only (Detector)

ZC3H12D Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240464C5

Cat no : 83507-2-PBS

Synonyms

MCP-induced protein 4, MCP induced protein 4, FLJ46041, EC:3.1.-.-, C6orf95

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Validation Data Gallery

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  WB analysis of Raji using 83507-2-RR (same clone as 83507-2-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 83507-2-RR (ZC3H12D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83507-2-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 83507-2-RR (same clone as 83507-2-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug ZC3H12D Recombinant antibody (83507-2-RR, Clone:240464C5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83507-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00495-1

  Cytometric bead array standard curve of MP00495-1, ZC3H12D Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83507-1-PBS. Detection antibody: 83507-2-PBS. Standard: Ag21770. Range: 0.156-20 ng/mL.

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  Affinity and Kinetic Characterization of 83507-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83507-2-RR against Human ZC3H12D were performed. The affinity constant is 0.343 nM.

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Product Information

83507-2-PBS targets ZC3H12D as part of a matched antibody pair:

MP00495-1: 83507-1-PBS capture and 83507-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ZC3H12D fusion protein Ag21770
• Full Name: zinc finger CCCH-type containing 12D
• Calculated Molecular Weight: 527 aa, 58 kDa
• GenBank Accession Number: BC157832
• Gene Symbol: ZC3H12D
• Gene ID (NCBI): 340152
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.