ZC3H12D Recombinant antibody

ZC3H12D Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240464C5

Cat no : 83507-2-RR

Synonyms

MCP-induced protein 4, MCP induced protein 4, FLJ46041, EC:3.1.-.-, C6orf95

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Validation Data Gallery

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  WB analysis of Raji using 83507-2-RR

  Raji cells were subjected to SDS PAGE followed by western blot with 83507-2-RR (ZC3H12D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  FC experiment of HeLa using 83507-2-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug ZC3H12D Recombinant antibody (83507-2-RR, Clone:240464C5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

• 

  Affinity and Kinetic Characterization of 83507-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83507-2-RR against Human ZC3H12D were performed. The affinity constant is 0.343 nM.

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Tested Applications

• Positive WB detected in: Raji cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83507-2-RR is ZC3H12D Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ZC3H12D fusion protein Ag21770
• Full Name: zinc finger CCCH-type containing 12D
• Calculated Molecular Weight: 527 aa, 58 kDa
• Observed Molecular Weight: 55-58 kDa
• GenBank Accession Number: BC157832
• Gene Symbol: ZC3H12D
• Gene ID (NCBI): 340152
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZC3H12D, also named as MCP induced protein 4, is a 527 amino acid protein, which contains one C3H1-type zinc finger and belongs to the ZC3H12 family. ZC3H12D exists as three isoforms and localizes in the cytoplasm. ZC3H12D may regulate cell growth likely by suppressing RB1 phosphorylation and serves as a tumor suppressor in certain leukemia cells. ZC3H12D is expressed in normal human lymphocytes but defective in some leukemia/lymphoma cell lines.