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ZC3H12D Recombinant antibody

ZC3H12D Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240464C5

Cat no : 83507-2-RR

Synonyms

MCP-induced protein 4, MCP induced protein 4, FLJ46041, EC:3.1.-.-, C6orf95

Validation Data Gallery

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  • Western Blot (WB) analysis of Raji cells using ZC3H12D Recombinant antibody (83507-2-RR)

    WB analysis of Raji using 83507-2-RR

    Raji cells were subjected to SDS PAGE followed by western blot with 83507-2-RR (ZC3H12D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of HeLa cells using ZC3H12D Recombinant antibody (83507-2-RR)

    FC experiment of HeLa using 83507-2-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug ZC3H12D Recombinant antibody (83507-2-RR, Clone:240464C5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83507-2-RR

    Affinity and Kinetic Characterization of 83507-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83507-2-RR against Human ZC3H12D were performed. The affinity constant is 0.343 nM.

Tested Applications

Positive WB detected inRaji cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83507-2-RR is ZC3H12D Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ZC3H12D fusion protein Ag21770
Full Name zinc finger CCCH-type containing 12D
Calculated Molecular Weight 527 aa, 58 kDa
Observed Molecular Weight 55-58 kDa
GenBank Accession NumberBC157832
Gene Symbol ZC3H12D
Gene ID (NCBI) 340152
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZC3H12D, also named as MCP induced protein 4, is a 527 amino acid protein, which contains one C3H1-type zinc finger and belongs to the ZC3H12 family. ZC3H12D exists as three isoforms and localizes in the cytoplasm. ZC3H12D may regulate cell growth likely by suppressing RB1 phosphorylation and serves as a tumor suppressor in certain leukemia cells. ZC3H12D is expressed in normal human lymphocytes but defective in some leukemia/lymphoma cell lines.

Protocols

Product Specific Protocols
WB protocol for ZC3H12D antibody 83507-2-RRDownload protocol
FC protocol for ZC3H12D antibody 83507-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols