CoraLite® Plus 488-conjugated ZC3H11A Recombinant antibody

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  IF Staining of HepG2 using CL488-82912-5

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 ZC3H11A antibody (CL488-82912-5, Clone: 230191D2 ) at dilution of 1:200.

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  FC experiment of HepG2 using CL488-82912-5

  1x10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated ZC3H11A Recombinant antibody (CL488-82912-5, Clone:230191D2)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

ZC3H11A Uni-rAb^TM Recombinant Antibody for IF/ICC, FC (Intra)

Cat No. CL488-82912-5

Clone No.230191D2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Synonyms

Zinc finger CCCH domain-containing protein 11A, ZC3HDC11A, KIAA0663, 230191D2

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-82912-5 targets ZC3H11A in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ZC3H11A fusion protein Ag23424
• Full Name: zinc finger CCCH-type containing 11A
• GenBank Accession Number: BC014268
• Gene Symbol: ZC3H11A
• Gene ID (NCBI): 9877
• RRID: AB_3673113
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O75152
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ZC3H11A is a RNA-binding protein that interacts with purine-rich sequences and is involved in nuclear mRNA export.