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CoraLite® Plus 488-conjugated ZC3H11A Recombinant antibody

ZC3H11A Recombinant Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

230191D2

Cat no : CL488-82912-5

Synonyms

Zinc finger CCCH domain-containing protein 11A, ZC3HDC11A, KIAA0663, 230191D2

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated ZC3H11A Recombinant (CL488-82912-5)

    IF Staining of HepG2 using CL488-82912-5

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 ZC3H11A antibody (CL488-82912-5, Clone: 230191D2 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite® Plus 488-conjugated ZC3H11A Recombinant (CL488-82912-5)

    FC experiment of HepG2 using CL488-82912-5

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated ZC3H11A Recombinant antibody (CL488-82912-5, Clone:230191D2)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL488-82912-5 is ZC3H11A Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ZC3H11A fusion protein Ag23424
Full Name zinc finger CCCH-type containing 11A
GenBank Accession NumberBC014268
Gene Symbol ZC3H11A
Gene ID (NCBI) 9877
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

ZC3H11A is a RNA-binding protein that interacts with purine-rich sequences and is involved in nuclear mRNA export.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 ZC3H11A antibody CL488-82912-5Download protocol
FC protocol for CL Plus 488 ZC3H11A antibody CL488-82912-5Download protocol
Standard Protocols
Click here to view our Standard Protocols