ChromoTek TurboGFP-Trap Magnetic Agarose, kit

TurboGFP-Trap Magnetic Agarose is an affinity reagent for immunoprecipitation (IP) of TurboGFP fusion proteins. It consists of an anti-TurboGFP VHH/ Nanobody that is coupled to magnetic agarose beads.

Specificity

TurboGFP, CopGFP, maxGFP

Applications

IP, CoIP

Type

Nanobody

Conjugate

Magnetic agarose

Publications

1

Cat no : tbtmak

Synonyms

TurboGFP-Trap, TurboGFP, CopGFP, maxGFP,maxFP-Green, green fluorescent protein CopGFP, TurboGFP tag

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Validation Data Gallery

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  Coomassie blue stained gel and Western blot.

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  TurboGFP-Trap Magnetic Agarose for pull-down of TurboGFP fusion proteins: Coomassie and Western Blot Input (I), non-bound (FT), and bound (B).

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Product Information

TurboGFP-Trap Magnetic Agarose is an affinity reagent for immunoprecipitation (IP) of TurboGFP fusion proteins. It consists of an anti-TurboGFP VHH/ Nanobody that is coupled to magnetic agarose beads.

• Description: The TurboGFP-Trap® Magnetic Agarose Kit contains TurboGFP-Trap Magnetic Agarose, lysis, wash, and elution buffers for efficient immunoprecipitation of TurboGFP-fusion proteins and their interacting factors.

  • Fast, reliable & efficient one-step immunoprecipitation

  • Ready-to-use

  • No heavy & light antibody chains on the SDS-PAGE

  • Stable under harsh washing conditions

  • Suitable for downstream mass spec analysis

• Applications: IP, CoIP
• Specificity/Target: TurboGFP, CopGFP, maxGFP, maxFP-Green ; For the complete list, please click here: Fluorescent protein specificity table
• Binding capacity: 7.5-10 μg of recombinant TurboGFP per 25 μL bead slurry
• Conjugate: Magnetic agarose beads; bead size: ~40 µm (cross-linked 6 % magnetic agarose beads)
• Elution buffer: SDS sample buffer; 0.1 mM citrate acid pH 3.0
• Wash buffer compatibility: 6 M urea, 2 M NaCl, 10 mM DTT, 2 % Nonidet P40 Substitute, 1 % Triton X-100, 0.2 % SDS
• Type: Nanobody
• Class: Recombinant
• Host: Alpaca
• Affinity (KD): Dissociation constant K_D of 0.5 nM
• Compatibility with mass spectrometry: The TurboGFP-Trap is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry
• RRID: AB_2827597
• Storage Buffer: 20% ethanol
• Storage Condition: Shipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze!

Kit components

Component	Description
TurboGFP-Trap Magnetic Agarose	20 reactions (500 µL)
Lysis buffer	Optimized for cytoplasmatic proteins and mammalian cell lysis
RIPA buffer	Optimized for nuclear/chromatin proteins and mammalian cell lysis
Wash buffer	Removal of unwanted proteins, peptides, etc.
Dilution buffer	Dilution of cell lysate
Elution buffer	For acidic elution

Other Formats

TurboGFP Trap Agarose kit

TurboGFP Trap Agarose

TurboGFP-Trap Magnetic Agarose

Publications

Application	Title
	Plant Biotechnol J; Fine-tuning the N-glycosylation of recombinant human erythropoietin using Chlamydomonas reinhardtii mutants; Authors - S Leprovost