CoraLite®594-conjugated TWIST2 Monoclonal antibody

TWIST2 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1B12D11

Cat no : CL594-66544

Synonyms

Dermo-1, DERMO1, Dermis-expressed protein 1, Class A basic helix-loop-helix protein 39, bHLHa39

---

Validation Data Gallery

• 

  IF Staining of HepG2 using CL594-66544

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594 TWIST2 antibody (CL594-66544, Clone: 1B12D11 ) at dilution of 1:200.

• 

  FC experiment of HeLa using CL594-66544

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human TWIST2 (CL594-66544, Clone:1B12D11) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Tested Applications

• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66544 targets TWIST2 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: TWIST2 fusion protein Ag2348
• Full Name: twist homolog 2 (Drosophila)
• Calculated Molecular Weight: 160 aa, 18 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC033168
• Gene Symbol: TWIST2
• Gene ID (NCBI): 117581
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

TWIST2, also named as BHLHA39 and DERMO1,is a basic helix-loop-helix protein which acts as a transcriptional regulator, plays a central role in differentiation of mesodermal during embryogenesis. TWIST2 inhibits the premature or ectopic differentiation of preosteoblast cells during osteogenesis, possibly by changing the internal signal transduction response of osteoblasts to external growth factors. It is also implicated in tumorigenesis, invasion and metastasis. TWIST2 forms both homodimers(45KD) and heterodimers with E2A E proteins, and dimer partner selection is a critical mediator of TWIST function. (PMID:14724576, 16502419).