CoraLite® Plus 488-conjugated TWIST2 Monoclonal antibody

TWIST2 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, mouse

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1B12D11

Cat no : CL488-66544

Synonyms

bHLHa39, Dermis expressed protein 1, Dermo 1, DERMO1, twist homolog 2 (Drosophila), Twist related protein 2, TWIST2

---

Validation Data Gallery

• 

  IF Staining of HepG2 using CL488-66544

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488-conjugated TWIST2 antibody (CL488-66544, Clone: 1B12D11 ) at dilution of 1:100.

---

Tested Applications

• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66544 targets TWIST2 in IF/ICC applications and shows reactivity with Human, rat, mouse samples.

• Tested Reactivity: Human, rat, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: TWIST2 fusion protein Ag2348
• Full Name: twist homolog 2 (Drosophila)
• Calculated Molecular Weight: 160 aa, 18 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC033168
• Gene Symbol: TWIST2
• Gene ID (NCBI): 117581
• RRID: AB_2919341
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

TWIST2, also named as BHLHA39 and DERMO1,is a basic helix-loop-helix protein which acts as a transcriptional regulator, plays a central role in differentiation of mesodermal during embryogenesis. TWIST2 inhibits the premature or ectopic differentiation of preosteoblast cells during osteogenesis, possibly by changing the internal signal transduction response of osteoblasts to external growth factors. It is also implicated in tumorigenesis, invasion and metastasis. TWIST2 forms both homodimers(45KD) and heterodimers with E2A E proteins, and dimer partner selection is a critical mediator of TWIST function. (PMID:14724576, 16502419).