Validation Data Gallery
|Positive WB detected in||HeLa cells|
|Positive IHC detected in||human breast cancer tissue, human ovary tumor tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:5000-1:20000|
|Immunohistochemistry (IHC)||IHC : 1:250-1:1000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
66544-1-Ig targets TWIST2 in WB, IHC, IF, ELISA applications and shows reactivity with Human, rat, mouse, pig samples.
|Tested Reactivity||Human, rat, mouse, pig|
|Host / Isotype||Mouse / IgG1|
|Immunogen||TWIST2 fusion protein Ag2348|
|Full Name||twist homolog 2 (Drosophila)|
|Calculated molecular weight||160 aa, 18 kDa|
|Observed molecular weight||22 kDa|
|GenBank accession number||BC033168|
|Gene ID (NCBI)||117581|
|Purification Method||Protein G purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
TWIST2, also named as BHLHA39 and DERMO1,is a basic helix-loop-helix protein which acts as a transcriptional regulator, plays a central role in differentiation of mesodermal during embryogenesis. TWIST2 inhibits the premature or ectopic differentiation of preosteoblast cells during osteogenesis, possibly by changing the internal signal transduction response of osteoblasts to external growth factors. It is also implicated in tumorigenesis, invasion and metastasis. TWIST2 forms both homodimers(45KD) and heterodimers with E2A E proteins, and dimer partner selection is a critical mediator of TWIST function. (PMID:14724576, 16502419).
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Expression of Dickkopf-1 and Twist2 in Cervical Squamous Cell Carcinoma and Their Correlation with Vasculogenic Mimicry.