TWIST2 Monoclonal antibody, PBS Only

TWIST2 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, mouse, pig

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B12D11

Cat no : 66544-1-PBS

Synonyms

bHLHa39, Dermis expressed protein 1, Dermo 1, DERMO1, twist homolog 2 (Drosophila), Twist related protein 2, TWIST2

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Validation Data Gallery

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  WB analysis using 66544-1-Ig (same clone as 66544-1-PBS)

  Various cells were subjected to SDS PAGE followed by western blot with 66544-1-Ig (TWIST2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66544-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66544-1-Ig (same clone as 66544-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66544-1-Ig (TWIST2 antibody) at dilution of 1:500 (under 40x lens). This data was developed using the same antibody clone with 66544-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 66544-1-Ig (same clone as 66544-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66544-1-Ig (TWIST2 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66544-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 66544-1-Ig (same clone as 66544-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66544-1-Ig (TWIST2 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66544-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66544-1-Ig (same clone as 66544-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66544-1-Ig (TWIST2 antibody) at dilution of 1:500 (under 10x lens). This data was developed using the same antibody clone with 66544-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66544-1-Ig (same clone as 66544-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TWIST2 antibody (66544-1-Ig, Clone: 1B12D11 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66544-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66544-1-Ig (same clone as 66544-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TWIST2 antibody (66544-1-Ig, Clone: 1B12D11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66544-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66544-1-PBS targets TWIST2 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, rat, mouse, pig samples.

• Tested Reactivity: Human, rat, mouse, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: TWIST2 fusion protein Ag2348
• Full Name: twist homolog 2 (Drosophila)
• Calculated Molecular Weight: 160 aa, 18 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC033168
• Gene Symbol: TWIST2
• Gene ID (NCBI): 117581
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

TWIST2, also named as BHLHA39 and DERMO1,is a basic helix-loop-helix protein which acts as a transcriptional regulator, plays a central role in differentiation of mesodermal during embryogenesis. TWIST2 inhibits the premature or ectopic differentiation of preosteoblast cells during osteogenesis, possibly by changing the internal signal transduction response of osteoblasts to external growth factors. It is also implicated in tumorigenesis, invasion and metastasis. TWIST2 forms both homodimers(45KD) and heterodimers with E2A E proteins, and dimer partner selection is a critical mediator of TWIST function. (PMID:14724576, 16502419).