Anti-Human TRAIL/CD253 Rabbit Recombinant Antibody, PBS Only

TRAIL/CD253 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

241419D2

Cat no : 98139-1-PBS

Synonyms

TNFSF10, TRAIL, TNFSF 10, TNF-related apoptosis-inducing ligand, Protein TRAIL

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Validation Data Gallery

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  FC experiment of human peripheral blood mononuclear cells using 98139-1-RR (same clone as 98139-1-PBS)

  1x10^6 untreated or IFN-alpha treated human peripheral blood mononuclear cells were surface stained with 0.25 ug Anti-Human TRAIL/CD253 Rabbit Recombinant Antibody (98139-1-RR, Clone: 241419D2) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Human CD14. Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were not fixed. This data was developed using the same antibody clone with 98139-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human peripheral blood mononuclear cells using 98139-1-RR (same clone as 98139-1-PBS)

  1x10^6 IFN-alpha treated human peripheral blood mononuclear cells were surface stained with 0.25 ug Anti-Human TRAIL/CD253 Rabbit Recombinant Antibody (98139-1-RR, Clone: 241419D2) (red) or Isotype Control (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated human peripheral blood mononuclear cells were surface stained with 0.25 ug Anti-Human TRAIL/CD253 Rabbit Recombinant Antibody (98139-1-RR, Clone: 241419D2) (black, dashed) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were not fixed. This data was developed using the same antibody clone with 98139-1-PBS in a different storage buffer formulation.

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Product Information

98139-1-PBS targets TRAIL/CD253 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant Protein
• Full Name: tumor necrosis factor (ligand) superfamily, member 10
• Calculated Molecular Weight: 281 aa, 33 kDa
• GenBank Accession Number: BC032722
• Gene Symbol: TRAIL
• Gene ID (NCBI): 8743
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

TRAIL, also known as CD253 or TNFSF10 (tumor necrosis factor superfamily member 10), is a typical death ligand expressed on natural killer cells and cytotoxic T lymphocytes. This protein preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues. TRAIL induces apoptotic cell death in cancer by binding to its functional death receptors, death receptor (DR) 4 (TNFRSF10A/TRAIL-R1) and DR5 (TNFRSF10B/TRAIL-R2) to activate the extrinsic apoptosis pathway. TRAIL also activates c-Jun N-terminal kinase (MAPK8/JNK) and the transcription factor nuclear factor-κB (NFκB). The binding of this protein to its receptors has been shown to trigger the activation of MAPK8/JNK, caspase 8, and caspase 3.