Anti-Human TRAIL/CD253 Rabbit Recombinant Antibody

TRAIL/CD253 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

241419D2

Cat no : 98139-1-RR

Synonyms

TNFSF10, TRAIL, TNFSF 10, TNF-related apoptosis-inducing ligand, Protein TRAIL

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Validation Data Gallery

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  FC experiment of human peripheral blood mononuclear cells using 98139-1-RR

  1x10^6 untreated or IFN-alpha treated human peripheral blood mononuclear cells were surface stained with 0.25 ug Anti-Human TRAIL/CD253 Rabbit Recombinant Antibody (98139-1-RR, Clone: 241419D2) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Human CD14. Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were not fixed.

• 

  FC experiment of human peripheral blood mononuclear cells using 98139-1-RR

  1x10^6 IFN-alpha treated human peripheral blood mononuclear cells were surface stained with 0.25 ug Anti-Human TRAIL/CD253 Rabbit Recombinant Antibody (98139-1-RR, Clone: 241419D2) (red) or Isotype Control (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated human peripheral blood mononuclear cells were surface stained with 0.25 ug Anti-Human TRAIL/CD253 Rabbit Recombinant Antibody (98139-1-RR, Clone: 241419D2) (black, dashed) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were not fixed.

• 

  Affinity and Kinetic Characterization of 98139-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98139-1-RR against Human TRAIL/CD253 were performed. The affinity constant is below 1 pM.

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Tested Applications

• Positive FC detected in: IFN-alpha treated human peripheral blood mononuclear cells

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98139-1-RR targets TRAIL/CD253 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant Protein
• Full Name: tumor necrosis factor (ligand) superfamily, member 10
• Calculated Molecular Weight: 281 aa, 33 kDa
• GenBank Accession Number: BC032722
• Gene Symbol: TRAIL
• Gene ID (NCBI): 8743
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

TRAIL, also known as CD253 or TNFSF10 (tumor necrosis factor superfamily member 10), is a typical death ligand expressed on natural killer cells and cytotoxic T lymphocytes. This protein preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues. TRAIL induces apoptotic cell death in cancer by binding to its functional death receptors, death receptor (DR) 4 (TNFRSF10A/TRAIL-R1) and DR5 (TNFRSF10B/TRAIL-R2) to activate the extrinsic apoptosis pathway. TRAIL also activates c-Jun N-terminal kinase (MAPK8/JNK) and the transcription factor nuclear factor-κB (NFκB). The binding of this protein to its receptors has been shown to trigger the activation of MAPK8/JNK, caspase 8, and caspase 3.