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  • Featured Product
  • KD/KO Validated

TPC1 Polyclonal antibody, PBS Only

TPC1 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 23758-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TPC1 Polyclonal antibody (23758-1-AP)

    WB analysis using 23758-1-AP (same clone as 23758-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 23758-1-AP (TPC1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23758-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TPC1 Polyclonal antibody (23758-1-AP)

    IF Staining of HepG2 using 23758-1-AP (same clone as 23758-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TPC1 antibody (23758-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 23758-1-PBS in a different storage buffer formulation.

Product Information

23758-1-PBS targets TPC1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TPC1 fusion protein Ag20607
Full Name two pore segment channel 1
Calculated Molecular Weight 816 aa, 94 kDa
Observed Molecular Weight 94 kDa
GenBank Accession NumberBC136795
Gene Symbol TPCN1
Gene ID (NCBI) 53373
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Two-pore channels (TPCs) are ancient members of the voltage-gated ion channel superfamily, are thought to be non-selective cation channels permeable to Ca2+. They mediate their physiological effects through releasing Ca2+ from acidic organelles in response to cues such as the second messenger, NAADP (nicotinic acid adenine dinucleotide phosphate). Genetic knockout and pharmacological inhibition experiments demonstrate that the two-pore channels, TPC1 and TPC2, are required for infection by Filoviruses Ebola and Marburg in mice.(PMID: 29746898; PMID 25722412) Two pore segment channel 1 (TPC1) is a human protein encoded by the TPCN1 gene.