TPC1 Polyclonal antibody

• Featured Product
• KD/KO Validated

TPC1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 23758-1-AP

Synonyms

TPCN1, Voltage-dependent calcium channel protein TPC1, two pore segment channel 1, Two pore channel protein 1, KIAA1169

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Validation Data Gallery

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  WB analysis using 23758-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23758-1-AP (TPC1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse heart using 23758-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 23758-1-AP (TPCN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 23758-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 23758-1-AP (TPCN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 23758-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TPC1 antibody (23758-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Tested Applications

• Positive WB detected in: mouse heart tissue, rat heart tissue
• Positive IHC detected in: mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below

Product Information

23758-1-AP targets TPC1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TPC1 fusion protein Ag20607
• Full Name: two pore segment channel 1
• Calculated Molecular Weight: 816 aa, 94 kDa
• Observed Molecular Weight: 94 kDa
• GenBank Accession Number: BC136795
• Gene Symbol: TPCN1
• Gene ID (NCBI): 53373
• RRID: AB_2879317
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Two-pore channels (TPCs) are ancient members of the voltage-gated ion channel superfamily, are thought to be non-selective cation channels permeable to Ca2+. They mediate their physiological effects through releasing Ca2+ from acidic organelles in response to cues such as the second messenger, NAADP (nicotinic acid adenine dinucleotide phosphate). Genetic knockout and pharmacological inhibition experiments demonstrate that the two-pore channels, TPC1 and TPC2, are required for infection by Filoviruses Ebola and Marburg in mice.(PMID: 29746898; PMID 25722412) Two pore segment channel 1 (TPC1) is a human protein encoded by the TPCN1 gene.

Publications

Species	Application	Title
mouse	WB	Sci Adv; CLN7 is an organellar chloride channel regulating lysosomal function.; Authors - Yayu Wang ; KD Validated
		PLoS Pathog; Pseudorabies virus inhibits progesterone-induced inactivation of TRPML1 to facilitate viral entry; Authors - Bing-Qian Su