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  • Featured Product
  • KD/KO Validated

TPC1 Polyclonal antibody

TPC1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 23758-1-AP

Synonyms

TPCN1, Voltage-dependent calcium channel protein TPC1, two pore segment channel 1, Two pore channel protein 1, KIAA1169

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TPC1 Polyclonal antibody (23758-1-AP)

    WB analysis using 23758-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23758-1-AP (TPC1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TPC1 Polyclonal antibody (23758-1-AP)

    IF Staining of HepG2 using 23758-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TPC1 antibody (23758-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

Tested Applications

Positive WB detected inmouse heart tissue, rat heart tissue
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

23758-1-AP targets TPC1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TPC1 fusion protein Ag20607
Full Name two pore segment channel 1
Calculated Molecular Weight 816 aa, 94 kDa
Observed Molecular Weight 94 kDa
GenBank Accession NumberBC136795
Gene Symbol TPCN1
Gene ID (NCBI) 53373
RRIDAB_2879317
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Two-pore channels (TPCs) are ancient members of the voltage-gated ion channel superfamily, are thought to be non-selective cation channels permeable to Ca2+. They mediate their physiological effects through releasing Ca2+ from acidic organelles in response to cues such as the second messenger, NAADP (nicotinic acid adenine dinucleotide phosphate). Genetic knockout and pharmacological inhibition experiments demonstrate that the two-pore channels, TPC1 and TPC2, are required for infection by Filoviruses Ebola and Marburg in mice.(PMID: 29746898; PMID 25722412) Two pore segment channel 1 (TPC1) is a human protein encoded by the TPCN1 gene.

Protocols

Product Specific Protocols
WB protocol for TPC1 antibody 23758-1-APDownload protocol
IF protocol for TPC1 antibody 23758-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Sci Adv

CLN7 is an organellar chloride channel regulating lysosomal function.

Authors - Yayu Wang
  • KD Validated
View Article

PLoS Pathog

Pseudorabies virus inhibits progesterone-induced inactivation of TRPML1 to facilitate viral entry

Authors - Bing-Qian Su
View Article