CoraLite® Plus 647-conjugated TNP1 Polyclonal antibody

TNP1 Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF-P

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat no : CL647-17178

Synonyms

STP 1, TNP1, TP 1, TP1

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Validation Data Gallery

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  IF Staining of mouse testis using CL647-17178

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite® Plus 647 TNP1 antibody (CL647-17178) at dilution of 1:50.

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  IF Staining of mouse testis using CL647-17178

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite® Plus 647 TNP1 antibody (CL647-17178) at dilution of 1:200, CoraLite®594 DAZL antibody (CL594-12633, red), CoraLite®488 BOULE antibody (CL488-13720, green).

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Tested Applications

• Positive IF-P detected in: mouse testis tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-17178 targets TNP1 in IF-P applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TNP1 fusion protein Ag10890
• Full Name: transition protein 1 (during histone to protamine replacement)
• Calculated Molecular Weight: 55 aa, 7 kDa
• Observed Molecular Weight: 6-10 kDa
• GenBank Accession Number: BC029516
• Gene Symbol: TNP1
• Gene ID (NCBI): 7141
• RRID: AB_2920233
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage.

Background Information

Transition nuclear proteins (TNP1 and TNP2) are the major nuclear proteins that replace somatic histones during spermatogenesis. TNPs are required for normal chromatin condensation and functional sperm development, spermatogenesis was found to be compromised in both Tnp1 and Tnp2 null mice. TNP1, or TP1, localized in nucleus, is a spermatid-specific product of the haploid genome which replaces histone and is itself replaced in the mature sperm by the protamines. Recently, TNP-1 was used as a germ cell marker in condensing spermatids.