TNP1 Polyclonal antibody
TNP1 Polyclonal Antibody for WB, IF-P, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF-P, ELISA
Conjugate
Unconjugated
Cat no : 17178-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse testis tissue |
| Positive IF-P detected in | mouse testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 9 publications below |
| IHC | See 3 publications below |
| IF | See 8 publications below |
Product Information
17178-1-AP targets TNP1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TNP1 fusion protein Ag10890 |
| Full Name | transition protein 1 (during histone to protamine replacement) |
| Calculated Molecular Weight | 55 aa, 7 kDa |
| Observed Molecular Weight | 6-10 kDa |
| GenBank Accession Number | BC029516 |
| Gene Symbol | TNP1 |
| Gene ID (NCBI) | 7141 |
| RRID | AB_2206757 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Transition nuclear proteins (TNP1 and TNP2) are the major nuclear proteins that replace somatic histones during spermatogenesis. TNPs are required for normal chromatin condensation and functional sperm development, spermatogenesis was found to be compromised in both Tnp1 and Tnp2 null mice. TNP1, or TP1, localized in nucleus, is a spermatid-specific product of the haploid genome which replaces histone and is itself replaced in the mature sperm by the protamines. Recently, TNP-1 was used as a germ cell marker in condensing spermatids.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TNP1 antibody 17178-1-AP | Download protocol |
| IF protocol for TNP1 antibody 17178-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Ubiquitination-Deficient Mutations in Human Piwi Cause Male Infertility by Impairing Histone-to-Protamine Exchange during Spermiogenesis. | ||
Mol Cell Chromodomain Protein CDYL Acts as a Crotonyl-CoA Hydratase to Regulate Histone Crotonylation and Spermatogenesis. | ||
J Hazard Mater Multi-omics analyses reveal the mechanisms of Arsenic-induced male reproductive toxicity in mice. | ||
Elife Absence of CEP78 causes photoreceptor and sperm flagella impairments in mice and a human individual | ||
Environ Pollut Silica nanoparticles induce spermatogenesis disorders via L3MBTL2-DNA damage-p53 apoptosis and RNF8-ubH2A/ubH2B pathway in mice. | ||
Environ Pollut Silica nanoparticles inhibiting the differentiation of round spermatid and chromatin remodeling of haploid period via MIWI in mice. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Chongil (Verified Customer) (08-19-2021) | This is a great IF antibody to detect Tnp1 protein.
 |
FH Zhenminm (Verified Customer) (07-08-2019) | This antibody is highly specific and works excellently for immunohistochemistry as well as immunofluorescence in formalin fixed and paraffin embedded tissue sections.
 |
