TLR5 Monoclonal antibody, PBS Only

TLR5 Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

pig, rat, mouse, human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

4B3D2

Cat no : 66570-1-PBS

Synonyms

Toll-like receptor 5, Toll/interleukin-1 receptor-like protein 3, toll like receptor 5, TIL3, SLEB1

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Validation Data Gallery

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  WB analysis of A549 using 66570-1-Ig (same clone as 66570-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 66570-1-Ig (TLR5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66570-1-PBS in a different storage buffer formulation.

• 

  WB analysis of THP-1 using 66570-1-Ig (same clone as 66570-1-PBS)

  THP-1 cells were subjected to SDS PAGE followed by western blot with 66570-1-Ig (TLR5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66570-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 66570-1-Ig (same clone as 66570-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66570-1-Ig (TLR5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66570-1-PBS in a different storage buffer formulation.

• 

  WB analysis of RAW 264.7 using 66570-1-Ig (same clone as 66570-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66570-1-Ig (TLR5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66570-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat spleen using 66570-1-Ig (same clone as 66570-1-PBS)

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 66570-1-Ig (TLR5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66570-1-PBS in a different storage buffer formulation.

• 

  IF Staining of THP-1 using 66570-1-Ig (same clone as 66570-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using TLR5 antibody (66570-1-Ig, Clone: 4B3D2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66570-1-PBS in a different storage buffer formulation.

• 

  IF Staining of THP-1 using 66570-1-Ig (same clone as 66570-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using TLR5 antibody (66570-1-Ig, Clone: 4B3D2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66570-1-PBS in a different storage buffer formulation.

• 

  FC experiment of THP-1 using 66570-1-Ig (same clone as 66570-1-PBS)

  1X10^6 THP-1 cells were intracellularly stained with 0.4 ug Anti-Human TLR5 (66570-1-Ig, Clone:4B3D2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66570-1-PBS in a different storage buffer formulation.

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Product Information

66570-1-PBS targets TLR5 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with pig, rat, mouse, human samples.

• Tested Reactivity: pig, rat, mouse, human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: TLR5 fusion protein Ag13840
• Full Name: toll-like receptor 5
• Calculated Molecular Weight: 858 aa, 98 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC109119
• Gene Symbol: TLR5
• Gene ID (NCBI): 7100
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

TLR5 belongs to the Toll-like receptor family which are important in the innate immune response to pathogens. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. TLR5 is known to recognize bacterial flagellin from invading mobile bacteria. TLR5 acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Mutations in TLR5 gene have been associated with both resistance and susceptibility to systemic lupus erythematosus, and susceptibility to Legionnaire disease.