TLR5 Monoclonal antibody

TLR5 Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

4B3D2

Cat no : 66570-1-Ig

Synonyms

Toll-like receptor 5, Toll/interleukin-1 receptor-like protein 3, toll like receptor 5, TIL3, SLEB1

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Validation Data Gallery

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  WB analysis of A549 using 66570-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 66570-1-Ig (TLR5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of THP-1 using 66570-1-Ig

  THP-1 cells were subjected to SDS PAGE followed by western blot with 66570-1-Ig (TLR5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 66570-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66570-1-Ig (TLR5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat spleen using 66570-1-Ig

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 66570-1-Ig (TLR5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66570-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66570-1-Ig (TLR5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of THP-1 using 66570-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using TLR5 antibody (66570-1-Ig, Clone: 4B3D2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of THP-1 using 66570-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using TLR5 antibody (66570-1-Ig, Clone: 4B3D2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of THP-1 using 66570-1-Ig

  1X10^6 THP-1 cells were intracellularly stained with 0.4 ug Anti-Human TLR5 (66570-1-Ig, Clone:4B3D2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: A549 cells, THP-1 cells, rat spleen tissue, HeLa cells, RAW 264.7 cells
• Positive IF/ICC detected in: THP-1 cells
• Positive FC (Intra) detected in: THP-1 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

66570-1-Ig targets TLR5 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Cited Reactivity: mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: TLR5 fusion protein Ag13840
• Full Name: toll-like receptor 5
• Calculated Molecular Weight: 858 aa, 98 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC109119
• Gene Symbol: TLR5
• Gene ID (NCBI): 7100
• RRID: AB_2881931
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TLR5 belongs to the Toll-like receptor family which are important in the innate immune response to pathogens. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. TLR5 is known to recognize bacterial flagellin from invading mobile bacteria. TLR5 acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Mutations in TLR5 gene have been associated with both resistance and susceptibility to systemic lupus erythematosus, and susceptibility to Legionnaire disease.

Publications

Species	Application	Title
mouse	WB	Nutr Diabetes; Gestational diabetes exacerbates intrauterine microbial exposure induced intestinal microbiota change in offspring contributing to increased immune response; Authors - Juncheng Liu