CoraLite® Plus 647-conjugated Synaptophysin Polyclonal antibody
Synaptophysin Polyclonal Antibody for IF-P, FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
IF-P, FC (Intra)
Conjugate
CoraLite® Plus 647 Fluorescent Dye
Cat no : CL647-17785
Synonyms
Validation Data Gallery
Tested Applications
Positive IF-P detected in | mouse pancreas tissue, mouse brain tissue |
Positive FC (Intra) detected in | PC-12 cells |
Recommended dilution
Application | Dilution |
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Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL647-17785 targets Synaptophysin in IF-P, FC (Intra) applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Synaptophysin fusion protein Ag11781 |
Full Name | synaptophysin |
Calculated Molecular Weight | 313 aa, 34 kDa |
Observed Molecular Weight | 38-40 kDa |
GenBank Accession Number | BC064550 |
Gene Symbol | Synaptophysin |
Gene ID (NCBI) | 6855 |
RRID | AB_2920235 |
Conjugate | CoraLite® Plus 647 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 654 nm / 674 nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. |
Background Information
Synaptophysin (SYP, also known as major synaptic vesicle protein p38) is a 38-kDa integral membrane glycoprotein that regulates synaptic vesicle endocytosis. It is the most abundant synaptic vesicle protein by mass. Synaptophysin is present in neuroendocrine cells and neurons that participate in synaptic transmission. Synaptophysin is a useful marker for identification of neuroendocrine cells and neoplasms. (PMID: 3010302; 21658579)
Protocols
Product Specific Protocols | |
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IF protocol for CL Plus 647 Synaptophysin antibody CL647-17785 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |