Synaptophysin Polyclonal antibody

Synaptophysin Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA

Conjugate

Unconjugated

Publications

205

Reviews (2)

Cat no : 17785-1-AP

Synonyms

SYP, SYN, MRX96

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Validation Data Gallery

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  WB analysis using 17785-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17785-1-AP (Synaptophysin antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17785-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17785-1-AP (Synaptophysin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse cerebellum using 17785-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 17785-1-AP (SYP antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 17785-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 17785-1-AP (SYP antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 17785-1-AP

  IP result of anti-Synaptophysin (IP:17785-1-AP, 3ug; Detection:17785-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

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  IHC staining of mouse brain using 17785-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17785-1-AP (Synaptophysin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 17785-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17785-1-AP (Synaptophysin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 17785-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 17785-1-AP (Synaptophysin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 17785-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 17785-1-AP (Synaptophysin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of ganglioma using 17785-1-AP

  Immunohistochemical analysis of paraffin-embedded ganglioma tissue slide using 17785-1-AP (Synaptophysin antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of ganglioma using 17785-1-AP

  Immunohistochemical analysis of paraffin-embedded ganglioma tissue slide using 17785-1-AP (Synaptophysin antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of neuroblastoma using 17785-1-AP

  Immunohistochemical analysis of paraffin-embedded neuroblastoma tissue slide using 17785-1-AP (Synaptophysin antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of neuroblastoma using 17785-1-AP

  Immunohistochemical analysis of paraffin-embedded neuroblastoma tissue slide using 17785-1-AP (Synaptophysin antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 17785-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 17785-1-AP (Synaptophysin antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human brain using 17785-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 17785-1-AP (Synaptophysin antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human pancreas using 17785-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 17785-1-AP (Synaptophysin antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human pancreas using 17785-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 17785-1-AP (Synaptophysin antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human pituitary adenoma using 17785-1-AP

  Immunohistochemical analysis of paraffin-embedded human pituitary adenoma tissue slide using 17785-1-AP (Synaptophysin antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human pituitary adenoma using 17785-1-AP

  Immunohistochemical analysis of paraffin-embedded human pituitary adenoma tissue slide using 17785-1-AP (Synaptophysin antibody at dilution of 1:50 (under 10x lens).

• 

  IF Staining of mouse pancreas using 17785-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using Synaptophysin antibody (17785-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse pancreas using 17785-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using Synaptophysin antibody (17785-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 17785-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Synaptophysin antibody (17785-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 17785-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Synaptophysin antibody (17785-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human lung cancer using 17785-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using 17785-1-AP (Synaptophysin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human lung cancer using 17785-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using 17785-1-AP (Synaptophysin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human gliomas using 17785-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human gliomas tissue using 17785-1-AP (Synaptophysin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human gliomas using 17785-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human gliomas tissue using 17785-1-AP (Synaptophysin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of PC-12 using 17785-1-AP

  Immunofluorescent analysis of PC-12 cells using 17785-1-AP (Synaptophysin antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse brain tissue, human brain tissue, mouse cerebellum tissue, PC-12 cells, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: mouse pancreas tissue, mouse brain tissue, human brain tissue, ganglioma tissue, human pancreas tissue, neuroblastoma tissue, human pituitary adenoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse pancreas tissue, human lung cancer tissue, human gliomas tissue, mouse brain tissue
• Positive IF/ICC detected in: PC-12 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 160 publications below
• IHC: See 22 publications below
• IF: See 51 publications below

Product Information

17785-1-AP targets Synaptophysin in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, canine, hamster
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Synaptophysin fusion protein Ag11781
• Full Name: synaptophysin
• Calculated Molecular Weight: 313 aa, 34 kDa
• Observed Molecular Weight: 38-40 kDa
• GenBank Accession Number: BC064550
• Gene Symbol: Synaptophysin
• Gene ID (NCBI): 6855
• RRID: AB_2271365
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Synaptophysin (SYP, also known as major synaptic vesicle protein p38) is a 38-kDa integral membrane glycoprotein that regulates synaptic vesicle endocytosis. It is the most abundant synaptic vesicle protein by mass. Synaptophysin is present in neuroendocrine cells and neurons that participate in synaptic transmission. Synaptophysin is a useful marker for the identification of neuroendocrine cells and neoplasms. Synaptic proteins including synaptophysin are reduced in brain tissue from patients with Alzheimer's disease.(PMID: 3010302; 21658579)

Publications

Species	Application	Title
human	IF	Nat Biotechnol; Magnify is a universal molecular anchoring strategy for expansion microscopy; Authors - Aleksandra Klimas
mouse, rat	WB,IF	Nat Genet; Identification of TMEM230 mutations in familial Parkinson's disease.; Authors - Han-Xiang Deng
mouse	WB,IF	Nat Neurosci; Aerobic glycolysis is the predominant means of glucose metabolism in neuronal somata, which protects against oxidative damage; Authors - Yao Wei
mouse	IF	Mol Cell; Mixed Lineage Kinase Domain-like Protein MLKL Breaks Down Myelin following Nerve Injury.; Authors - Zhengxin Ying
mouse	WB	Neuron; Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory.; Authors - Xiaohui Li
mouse	IF	Neuron; Loss of PYCR2 Causes Neurodegeneration by Increasing Cerebral Glycine Levels via SHMT2.; Authors - Nathalie Escande-Beillard

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Alexandra (Verified Customer) (06-11-2023)

Hoechst-blue Green-Tubulin III (neuronal marker) Orange (Synaptophysin)

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:300
• Cell Tissue Type: P19 derived neurons

Synaptophysin Antibody Immunofluorescence validation (1:300 dilution) in P19 derived neurons (Cat no:17785-1-AP)

FH

Azita (Verified Customer) (05-31-2021)

Human primary cortical neurons was labelled with 1/1000 of the Synaptophysin Polyclonal antibody. The labelling was strong and very good. Blue (Bisbenzimide) Green (Synaptophysin)

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Human primary cortical neurons

Synaptophysin Antibody Immunohistochemistry validation (1/1000 dilution) in Human primary cortical neurons (Cat no:17785-1-AP)

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