Tested Applications
| Positive WB detected in | mouse brain tissue, PC-12 cells, rat brain tissue, mouse cerebellum tissue, rat cerebellum tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | mouse pancreas tissue, mouse brain tissue, human brain tissue, ganglioma tissue, human pancreas tissue, neuroblastoma tissue, human pituitary adenoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse pancreas tissue, human lung cancer tissue, human gliomas tissue, mouse brain tissue |
| Positive IF/ICC detected in | PC-12 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:20000-1:100000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 188 publications below |
| IHC | See 24 publications below |
| IF | See 59 publications below |
Product Information
17785-1-AP targets Synaptophysin in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, canine, hamster |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synaptophysin fusion protein Ag11781 Predict reactive species |
| Full Name | synaptophysin |
| Calculated Molecular Weight | 313 aa, 34 kDa |
| Observed Molecular Weight | 38-40 kDa |
| GenBank Accession Number | BC064550 |
| Gene Symbol | Synaptophysin |
| Gene ID (NCBI) | 6855 |
| RRID | AB_2271365 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P08247 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Synaptophysin (SYP, also known as major synaptic vesicle protein p38) is a 38-kDa integral membrane glycoprotein that regulates synaptic vesicle endocytosis. It is the most abundant synaptic vesicle protein by mass. Synaptophysin is present in neuroendocrine cells and neurons that participate in synaptic transmission. Synaptophysin is a useful marker for the identification of neuroendocrine cells and neoplasms. Synaptic proteins including synaptophysin are reduced in brain tissue from patients with Alzheimer's disease.(PMID: 3010302; 21658579)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Synaptophysin antibody 17785-1-AP | Download protocol |
| IHC protocol for Synaptophysin antibody 17785-1-AP | Download protocol |
| IF protocol for Synaptophysin antibody 17785-1-AP | Download protocol |
| IP protocol for Synaptophysin antibody 17785-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Biotechnol Magnify is a universal molecular anchoring strategy for expansion microscopy | ||
Nat Neurosci Aerobic glycolysis is the predominant means of glucose metabolism in neuronal somata, which protects against oxidative damage | ||
Mol Cell Mixed Lineage Kinase Domain-like Protein MLKL Breaks Down Myelin following Nerve Injury. | ||
Neuron Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory. | ||
Neuron Loss of PYCR2 Causes Neurodegeneration by Increasing Cerebral Glycine Levels via SHMT2. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Alexandra (Verified Customer) (06-11-2023) | Hoechst-blue Green-Tubulin III (neuronal marker) Orange (Synaptophysin)
 |
FH Azita (Verified Customer) (05-31-2021) | Human primary cortical neurons was labelled with 1/1000 of the Synaptophysin Polyclonal antibody. The labelling was strong and very good. Blue (Bisbenzimide) Green (Synaptophysin)
 |
