CoraLite® Plus 488-conjugated Synaptophysin Polyclonal antibody

Synaptophysin Polyclonal Antibody for IF-P

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-17785

Synonyms

synaptophysin, SYP

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Validation Data Gallery

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  IF Staining of mouse pancreas using CL488-17785

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using CoraLite® Plus 488 Synaptophysin antibody (CL488-17785) at dilution of 1:200.

• 

  IF Staining of mouse pancreas using CL488-17785

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using CoraLite® Plus 488 Synaptophysin antibody (CL488-17785) at dilution of 1:200.

• 

  IF Staining of mouse brain using CL488-17785

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 Synaptophysin antibody (CL488-17785) at dilution of 1:1000.

• 

  IF Staining of mouse brain using CL488-17785

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 Synaptophysin antibody (CL488-17785) at dilution of 1:1000.

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Tested Applications

• Positive IF-P detected in: mouse pancreas tissue, mouse brain tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-17785 targets Synaptophysin in IF-P applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Synaptophysin fusion protein Ag11781
• Full Name: synaptophysin
• Calculated Molecular Weight: 313 aa, 34 kDa
• Observed Molecular Weight: 38-40 kDa
• GenBank Accession Number: BC064550
• Gene Symbol: Synaptophysin
• Gene ID (NCBI): 6855
• RRID: AB_2919155
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Synaptophysin (SYP, also known as major synaptic vesicle protein p38) is a 38-kDa integral membrane glycoprotein that regulates synaptic vesicle endocytosis. It is the most abundant synaptic vesicle protein by mass. Synaptophysin is present in neuroendocrine cells and neurons that participate in synaptic transmission. Synaptophysin is a useful marker for identification of neuroendocrine cells and neoplasms. (PMID: 3010302; 21658579)