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CoraLite®594-conjugated Synaptophysin Monoclonal antibody

Synaptophysin Monoclonal Antibody for IF-P, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

IF-P, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

4E12C4

Cat no : CL594-67864

Synonyms

SYP, SYN, MRX96, 4E12C4

Validation Data Gallery

Filter:
  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CoraLite®594-conjugated Synaptophysin Monoclonal a (CL594-67864)

    IF Staining of mouse brain using CL594-67864

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite®594 Synaptophysin antibody (CL594-67864, Clone: 4E12C4 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CoraLite®594-conjugated Synaptophysin Monoclonal a (CL594-67864)

    IF Staining of mouse brain using CL594-67864

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite®594 Synaptophysin antibody (CL594-67864, Clone: 4E12C4 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CoraLite®594-conjugated Synaptophysin Monoclonal a (CL594-67864)

    IF Staining of mouse brain using CL594-67864

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite®594 Synaptophysin antibody (CL594-67864, Clone: 4E12C4 ) at dilution of 1:2000.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CoraLite®594-conjugated Synaptophysin Monoclonal a (CL594-67864)

    IF Staining of mouse brain using CL594-67864

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite®594 Synaptophysin antibody (CL594-67864, Clone: 4E12C4 ) at dilution of 1:2000.

  • Flow cytometry (FC) experiment of PC-12 cells using CoraLite®594-conjugated Synaptophysin Monoclonal a (CL594-67864)

    FC experiment of PC-12 using CL594-67864

    1X10^6 PC-12 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human Synaptophysin (CL594-67864, Clone:4E12C4) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL594-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF-P detected inmouse brain tissue
Positive FC (Intra) detected inPC-12 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67864 targets Synaptophysin in IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Synaptophysin fusion protein Ag11803
Full Name synaptophysin
Calculated Molecular Weight 313 aa, 34 kDa
GenBank Accession NumberBC064550
Gene Symbol Synaptophysin
Gene ID (NCBI) 6855
RRIDAB_2920187
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Synaptophysin (SYP, also known as major synaptic vesicle protein p38) is a 38-kDa integral membrane glycoprotein that regulates synaptic vesicle endocytosis. It is the most abundant synaptic vesicle protein by mass. Synaptophysin is present in neuroendocrine cells and neurons that participate in synaptic transmission. Synaptophysin is a useful marker for identification of neuroendocrine cells and neoplasms. (PMID: 3010302; 21658579)

Protocols

Product Specific Protocols
IF protocol for CL594 Synaptophysin antibody CL594-67864Download protocol
Standard Protocols
Click here to view our Standard Protocols