CoraLite® Plus 488-conjugated Synaptophysin Monoclonal antibody
Synaptophysin Monoclonal Antibody for IF-P, FC (Intra)
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat, pig
Applications
IF-P, FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
4E12C4
Cat no : CL488-67864
Synonyms
Validation Data Gallery
Tested Applications
Positive IF-P detected in | mouse brain tissue |
Positive FC (Intra) detected in | PC-12 cells |
Recommended dilution
Application | Dilution |
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Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL488-67864 targets Synaptophysin in IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.
Tested Reactivity | human, mouse, rat, pig |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | Synaptophysin fusion protein Ag11803 |
Full Name | synaptophysin |
Calculated Molecular Weight | 313 aa, 34 kDa |
GenBank Accession Number | BC064550 |
Gene Symbol | Synaptophysin |
Gene ID (NCBI) | 6855 |
RRID | AB_2909472 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Synaptophysin (SYP, also known as major synaptic vesicle protein p38) is a 38-kDa integral membrane glycoprotein that regulates synaptic vesicle endocytosis. It is the most abundant synaptic vesicle protein by mass. Synaptophysin is present in neuroendocrine cells and neurons that participate in synaptic transmission. Synaptophysin is a useful marker for identification of neuroendocrine cells and neoplasms. (PMID: 3010302; 21658579)
Protocols
Product Specific Protocols | |
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IF protocol for CL Plus 488 Synaptophysin antibody CL488-67864 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |