Anti-Human Siglec-7/CD328 Rabbit Recombinant Antibody, PBS Only

Siglec-7/CD328 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

241525H1

Cat no : 98183-1-PBS

Synonyms

SIGLEC7, Siglec-7, D siglec, CD328, AIRM-1

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Validation Data Gallery

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  FC experiment of human peripheral blood leukocytes using 98183-1-RR (same clone as 98183-1-PBS)

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human Siglec-7/CD328 Rabbit Recombinant Antibody (98183-1-RR, Clone: 241525H1) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) and PE-conjugated Goat Anti-Rabbit IgG. Cells were then stained with CoraLite® Plus 647 Anti-Human NCAM1/CD56. Cells were not fixed. This data was developed using the same antibody clone with 98183-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human peripheral blood leukocytes using 98183-1-RR (same clone as 98183-1-PBS)

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human Siglec-7/CD328 Rabbit Recombinant Antibody (98183-1-RR, Clone:241525H1) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue) and PE-conjugated Goat Anti-Rabbit IgG. Cells were not fixed. This data was developed using the same antibody clone with 98183-1-PBS in a different storage buffer formulation.

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Product Information

The immunogen of 98183-1-PBS is Siglec-7/CD328 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: sialic acid binding Ig-like lectin 7
• Calculated Molecular Weight: 51kDa
• GenBank Accession Number: NM_014385.4
• Gene Symbol: Siglec-7
• Gene ID (NCBI): 27036
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Sialic acid binding Ig-like lectin 7 (Siglec-7), also known as CD328 or p75/AIRM-1, is a member of the Siglec family of glycan-recognition proteins. Siglec-7 is a type-I transmembrane protein consisting of three extracellular immunoglobulin-like domains that comprise an N-terminal V-set domain and two C2-set domains, a transmembrane region and a cytoplasmic tail containing two tyrosine residues embodied in immunoreceptor tyrosine-based inhibition motif-like motifs (PMID: 32322597; 10567377). It is mainly expressed on immune cells, with low levels on granulocytes, intermediate levels on monocytes, and relatively high levels on a major subset of natural killer cells and a minor subset of CD8+ T cells (PMID: 10567377). Siglec-7 is an inhibitory receptor that negatively regulates the function of NK cells and modulates the immune response through the interaction of sialic acid-containing ligands (PMID: 27312286).