SUN2 Recombinant antibody

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  WB analysis using 85103-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85103-3-RR (UNC84B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 85103-3-RR

  HeLa cells were subjected to SDS PAGE followed by western blot with 85103-3-RR (UNC84B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 85103-3-RR

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 85103-3-RR (SUN2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HEPG2 using 85103-3-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SUN2 antibody (85103-3-RR, Clone: 242533G3 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of HeLa using 85103-3-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug SUN2 Recombinant antibody (85103-3-RR, Clone:242533G3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  Affinity and Kinetic Characterization of 85103-3-RR

  Biolayer interferometry (BLl) kinetic assays of 85103-3-RR against Human SUN2 were performed. The affinity constant is 23.7 pM.

SUN2 {ptg:Brand} Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Cat No. 85103-3-RR

Clone No.242533G3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

UNC84B, 242533G3, FRIGG, KIAA0668, Protein unc-84 homolog B

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Tested Applications

• Positive WB detected in: Jurkat cells, HeLa cells, Saos-2 cells
• Positive IHC detected in: human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85103-3-RR targets SUN2 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SUN2 fusion protein Ag16077
• Full Name: unc-84 homolog B (C. elegans)
• Calculated Molecular Weight: 717 aa, 80 kDa
• Observed Molecular Weight: 80-85 kDa
• GenBank Accession Number: BC111717
• Gene Symbol: SUN2
• Gene ID (NCBI): 25777
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9UH99
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SUN2, also known as UNC84B, RAB5IP, belongs to the SUN (Sad1 and UNC84) protein family, which is a group of conserved proteins in eukaryotes. SUN2 is a key component of the LINC (Linker of Nucleoskeleton and Cytoskeleton) complex, which connects the nuclear lamina to the cytoskeleton. This connection is crucial for the transmission of mechanical forces across the nuclear envelope and for nuclear movement and positioning (PMID: 18396275).