SUN2 Recombinant antibody, PBS Only (Capture)

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  WB analysis using 85103-3-RR (same clone as 85103-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85103-3-RR (UNC84B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85103-3-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 85103-3-RR (same clone as 85103-3-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 85103-3-RR (UNC84B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85103-3-PBS in a different storage buffer formulation.

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  IF Staining of HEPG2 using 85103-3-RR (same clone as 85103-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SUN2 antibody (85103-3-RR, Clone: 242533G3 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85103-3-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01849-2

  Cytometric bead array standard curve of MP01849-2, SUN2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85103-3-PBS. Detection antibody: 85103-2-PBS. Standard: Ag16077. Range: 1.25-80 ng/mL.

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  Cytometric bead array standard curve of MP01849-1

  Cytometric bead array standard curve of MP01849-1, SUN2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85103-3-PBS. Detection antibody: 85103-1-PBS. Standard: Ag16077. Range: 0.625-80 ng/mL.

SUN2 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Cat No. 85103-3-PBS

Clone No.242533G3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Synonyms

UNC84B, Sad1/unc-84 protein-like 2, Rab5-interacting protein, Protein unc-84 homolog B, KIAA0668

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Product Information

85103-3-PBS targets SUN2 as part of a matched antibody pair:

MP01849-1: 85103-3-PBS capture and 85103-1-PBS detection (validated in Cytometric bead array)

MP01849-2: 85103-3-PBS capture and 85103-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SUN2 fusion protein Ag16077
• Full Name: unc-84 homolog B (C. elegans)
• Calculated Molecular Weight: 717 aa, 80 kDa
• Observed Molecular Weight: 80-85 kDa
• GenBank Accession Number: BC111717
• Gene Symbol: SUN2
• Gene ID (NCBI): 25777
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9UH99
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

SUN2, also known as UNC84B, RAB5IP, belongs to the SUN (Sad1 and UNC84) protein family, which is a group of conserved proteins in eukaryotes. SUN2 is a key component of the LINC (Linker of Nucleoskeleton and Cytoskeleton) complex, which connects the nuclear lamina to the cytoskeleton. This connection is crucial for the transmission of mechanical forces across the nuclear envelope and for nuclear movement and positioning (PMID: 18396275).