CoraLite®594-conjugated SUMO2/3 Monoclonal antibody

• Featured Product
• KD/KO Validated

SUMO2/3 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1A1B3

Cat no : CL594-67154

Synonyms

SUMO2, Small ubiquitin-related modifier 2, Sentrin-2, Sentrin2, Sentrin 2

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Validation Data Gallery

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  IF Staining of HeLa using CL594-67154

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CL594-67154 (SUMO2/3 antibody) at dilution of 1:100.

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  IF Staining of HeLa using CL594-67154

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 SUMO2/3 antibody (CL594-67154, Clone: 1A1B3 ) at dilution of 1:200.

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  FC experiment of HeLa using CL594-67154

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human SUMO2/3 (CL594-67154, Clone:1A1B3) (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67154 targets SUMO2/3 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: SUMO2/3 fusion protein Ag28672
• Full Name: SMT3 suppressor of mif two 3 homolog 2 (S. cerevisiae)
• Calculated Molecular Weight: 11 kDa
• Observed Molecular Weight: 18 kDa
• GenBank Accession Number: BC008450
• Gene Symbol: SUMO2
• Gene ID (NCBI): 6613
• RRID: AB_2883641
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Ubiquitin is most famous for its function in targeting proteins for degradation by the 26S proteasome, ubiquitin needs to be attached to a substrate in chains (polyubiquitylation) before being recognized by proteasome. Similarly, SUMO (small ubiquitin-related modifier) can be linked to substrates in chains (polysumoylation), SUMO modification has been implicated in many important cellular processes including the control of genome stability, signal transduction, targeting to and formation of nuclear compartments, cell cycle and meiosis. There are 4 confirmed SUMO isoforms in human, SUMO-1, SUMO-2, SUMO-3 and SUMO-4. SUMO-2 and SUMO-3 are nearly identical but are distinct from SUMO-1. SUMO2/3 conjugation was recently widely involved in neuroprotective activities. A substitution (M55V) of SUMO4 was strongly associated with the pathogenesis of type 1 diabetes (T1D) involving NF kappa B related mechanisms.

Related Products

Foxp3 / Transcription Factor Staining Buffer Kit

PF00011