SUMO2/3 Monoclonal antibody

• Featured Product
• KD/KO Validated

SUMO2/3 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

CloneNo.

1A1B3

Cat no : 67154-1-Ig

Synonyms

SUMO2, Small ubiquitin-related modifier 2, Sentrin-2, Sentrin2, Sentrin 2

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Validation Data Gallery

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  WB analysis of HEK-293 using 67154-1-Ig

  WB result of SUMO2/3 antibody (67154-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SUMO2/3 transfected HEK-293 cells.

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  WB analysis using 67154-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67154-1-Ig (SUMO2/3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 67154-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 67154-1-Ig (SUMO2/3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 67154-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67154-1-Ig (SUMO2/3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 67154-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67154-1-Ig (SUMO2/3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 67154-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67154-1-Ig (SUMO2/3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 67154-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67154-1-Ig (SUMO2/3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 67154-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67154-1-Ig (SUMO2/3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 67154-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SUMO2/3 antibody (67154-1-Ig, Clone: 1A1B3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red).

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  IF Staining of HeLa using 67154-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SUMO2/3 antibody (67154-1-Ig, Clone: 1A1B3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, Jurkat cells, A549 cells, NIH/3T3 cells, K-562 cells, HSC-T6 cells, PC-12 cells
• Positive IHC detected in: human prostate cancer tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

67154-1-Ig targets SUMO2/3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: SUMO2/3 fusion protein Ag28672
• Full Name: SMT3 suppressor of mif two 3 homolog 2 (S. cerevisiae)
• Calculated Molecular Weight: 11 kDa
• Observed Molecular Weight: 18 kDa
• GenBank Accession Number: BC008450
• Gene Symbol: SUMO2
• Gene ID (NCBI): 6613
• RRID: AB_2882451
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ubiquitin is most famous for its function in targeting proteins for degradation by the 26S proteasome, ubiquitin needs to be attached to a substrate in chains (polyubiquitylation) before being recognized by proteasome. Similarly, SUMO (small ubiquitin-related modifier) can be linked to substrates in chains (polysumoylation), SUMO modification has been implicated in many important cellular processes including the control of genome stability, signal transduction, targeting to and formation of nuclear compartments, cell cycle and meiosis. There are 4 confirmed SUMO isoforms in human, SUMO-1, SUMO-2, SUMO-3 and SUMO-4. SUMO-2 and SUMO-3 are nearly identical but are distinct from SUMO-1. SUMO2/3 conjugation was recently widely involved in neuroprotective activities. A substitution (M55V) of SUMO4 was strongly associated with the pathogenesis of type 1 diabetes (T1D) involving NF kappa B related mechanisms.

Publications

Species	Application	Title
human	WB	Nat Commun; BioE3 identifies specific substrates of ubiquitin E3 ligases; Authors - Orhi Barroso-Gomila
human	WB	Nat Cell Biol; Transcription-coupled DNA-protein crosslink repair by CSB and CRL4CSA-mediated degradation; Authors - Marjolein van Sluis