STAT1 Recombinant antibody, PBS Only

• Featured Product
• KD/KO Validated

STAT1 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2F16

Cat no : 82016-1-PBS

Synonyms

Transcription factor ISGF-3 components p91/p84, STAT91, Signal transducer and activator of transcription 1-alpha/beta, ISGF 3, 2F16

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Validation Data Gallery

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  WB analysis using 82016-1-RR (same clone as 82016-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82016-1-RR (STAT1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82016-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 82016-1-RR (same clone as 82016-1-PBS)

  WB result of STAT1 antibody (82016-1-RR; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-STAT1 transfected HEK-293 cells. This data was developed using the same antibody clone with 82016-1-PBS in a different storage buffer formulation.

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  IP experiment of PC-3 using 82016-1-RR (same clone as 82016-1-PBS)

  IP result of anti-STAT1 (IP:82016-1-RR, 4ug; Detection:82016-1-RR 1:10000) with PC-3 cells lysate 2000 ug. This data was developed using the same antibody clone with 82016-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 82016-1-RR (same clone as 82016-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 82016-1-RR (STAT1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82016-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 82016-1-RR (same clone as 82016-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 82016-1-RR (STAT1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82016-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 82016-1-RR (same clone as 82016-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 82016-1-RR (STAT1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82016-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 82016-1-RR (same clone as 82016-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 82016-1-RR (STAT1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82016-1-PBS in a different storage buffer formulation.

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  IHC staining of rat colon using 82016-1-RR (same clone as 82016-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 82016-1-RR (STAT1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82016-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat spleen using 82016-1-RR (same clone as 82016-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 82016-1-RR (STAT1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82016-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 82016-1-RR (same clone as 82016-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed IFN alpha treated HeLa cells using STAT1 antibody (82016-1-RR, Clone: 2F16 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 82016-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82016-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82016-1-RR against Human STAT1 were performed. The affinity constant is 0.501 nM.

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Product Information

82016-1-PBS targets STAT1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: STAT1 fusion protein Ag0199
• Full Name: signal transducer and activator of transcription 1, 91kDa
• Calculated Molecular Weight: 83 kDa
• Observed Molecular Weight: 84 kDa
• GenBank Accession Number: BC002704
• Gene Symbol: STAT1
• Gene ID (NCBI): 6772
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

STAT1 (signal transducers and activators of transcription 1) is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. Various ligands, including interferons- and - , EGF, PDGF and IL-6, can activate STAT1. STAT1 protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens.