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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated SP1 Polyclonal antibody

SP1 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-21962

Synonyms

TSFP1, Specificity protein 1, Sp1 transcription factor, SP 1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite®594-conjugated SP1 Polyclonal antibody (CL594-21962)

    IF Staining of HepG2 using CL594-21962

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594 SP1 antibody (CL594-21962) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite®594-conjugated SP1 Polyclonal antibody (CL594-21962)

    FC experiment of HeLa using CL594-21962

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human SP1 (CL594-21962) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-21962 targets SP1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, monkey samples.

Tested Reactivity human, mouse, rat, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SP1 fusion protein Ag16720
Full Name Sp1 transcription factor
Calculated Molecular Weight 785 aa, 81 kDa
Observed Molecular Weight 95-106 kDa
GenBank Accession NumberBC062539
Gene Symbol SP1
Gene ID (NCBI) 6667
RRIDAB_2919865
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

The transcription factor Sp1 is a C2H2 zinc-finger protein that is involved in the regulation of a wide variety of genes, including housekeeping genes and tumor-developing genes. It is associated with tumor development, growth, and metastasis [PMID: 16209919]. It regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Besides, it has a role in modulating the cellular response to DNA damage, recruiting SMARCA4/BRG1 on the c-FOS promoter, regulation of FE65 gene expression [PMID:11371615,16332679]. SP1 is detected with MW 95-105 kDa and 65 kDa in this paper(PMID: 10618488).

Protocols

Product Specific Protocols
IF protocol for CL594 SP1 antibody CL594-21962Download protocol
FC protocol for CL594 SP1 antibody CL594-21962Download protocol
Standard Protocols
Click here to view our Standard Protocols