CoraLite®594-conjugated SMARCA4 Monoclonal antibody

SMARCA4 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2E6B6

Cat no : CL594-66561

Synonyms

BRG1, BAF190A, BAF190, ATP-dependent helicase SMARCA4, ATP dependent helicase SMARCA4

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Validation Data Gallery

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  IF Staining of HepG2 using CL594-66561

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CL594-66561 (SMARCA4 antibody) at dilution of 1:50.

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  IF Staining of HepG2 using CL594-66561

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594 SMARCA4 antibody (CL594-66561, Clone: 2E6B6 ) at dilution of 1:200. DAPI (blue).

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  FC experiment of HeLa using CL594-66561

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite®594 Anti-Human SMARCA4 (CL594-66561, Clone:2E6B6) (red), or 0.2 ug Mouse IgG2b Isotype Control (CL594-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66561 targets SMARCA4 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: SMARCA4 fusion protein Ag16256
• Full Name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4
• Calculated Molecular Weight: 1647 aa, 185 kDa
• GenBank Accession Number: BC150298
• Gene Symbol: SMARCA4
• Gene ID (NCBI): 6597
• RRID: AB_2883603
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

SMARCA4, also named as BAF190A, BRG1, SNF2B and SNF2L4, belongs to the SNF2/RAD54 helicase family. SMARCA4 is a transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. It is a component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. It is also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. And this anitbody is CL594(Ex/Em 593 nm/614 nm) conjugated.

Related Products

Foxp3 / Transcription Factor Staining Buffer Kit

PF00011