• Featured Product
  • KD/KO Validated

SMARCA4/BRG1 Monoclonal antibody

SMARCA4/BRG1 Monoclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, rat, mouse and More (1)

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

CloneNo.

2E6B6

Cat no : 66561-1-Ig

Synonyms

ATP dependent helicase SMARCA4, BAF190, BAF190A, BRG1, BRG1 associated factor 190A, hSNF2b, Protein brahma homolog 1, Protein BRG 1, SMARCA4, SMARCA4/BRG1, SNF2, SNF2 BETA, SNF2B, SNF2L4, SNF2LB, SWI2, Transcription activator BRG1



Tested Applications

Positive WB detected inHepG2 cells, COLO 320 cells, HeLa cells, Jurkat cells, K-562 cells, HSC-T6 cells, 4T1 cells
Positive IP detected inHeLa cells
Positive IF/ICC detected inHepG2 cells, PC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66561-1-Ig targets SMARCA4/BRG1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with Human, rat, mouse samples.

Tested Reactivity Human, rat, mouse
Cited Reactivityhuman
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen SMARCA4/BRG1 fusion protein Ag16256
Full Name SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4
Calculated Molecular Weight 1647 aa, 185 kDa
Observed Molecular Weight 185 kDa
GenBank Accession NumberBC150298
Gene Symbol SMARCA4
Gene ID (NCBI) 6597
RRIDAB_2881922
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SMARCA4, also named as BAF190A, BRG1, SNF2B and SNF2L4, belongs to the SNF2/RAD54 helicase family. SMARCA4 is a transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. It is a component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. It is also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.

Protocols

Product Specific Protocols
WB protocol for SMARCA4/BRG1 antibody 66561-1-IgDownload protocol
IF protocol for SMARCA4/BRG1 antibody 66561-1-IgDownload protocol
IP protocol for SMARCA4/BRG1 antibody 66561-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Inflammation

Regulation of KDM2B and Brg1 on Inflammatory Response of Nasal Mucosa in CRSwNP.

Authors - C C Liu
  • KD Validated