SMARCA4 Recombinant antibody, PBS Only (Detector)

SMARCA4 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240172F4

Cat no : 83310-2-PBS

Synonyms

BRG1, BAF190A, BAF190, ATP-dependent helicase SMARCA4, ATP dependent helicase SMARCA4

---

Validation Data Gallery

• 

  WB analysis using 83310-2-RR (same clone as 83310-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83310-2-RR (SMARCA4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83310-2-PBS in a different storage buffer formulation.

• 

  Sandwich ELISA standard curve of MP00334-1

  Sandwich ELISA standard curve of MP00334-1, Human SMARCA4 Recombinant Matched Antibody Pair - PBS only. 83310-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag16256. 83310-2-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

• 

  Sample test of MP00334-1

  The mean SMARCA4 concentration was determined to be 22.1 ng/mL in Hela cell extract based on a 3.6 mg/mL extract load and 6.9 ng/mL in HEK-293 cell extract based on a 4.6 mg/mL extract load.

• 

  IF Staining of HepG2 using 83310-2-RR (same clone as 83310-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SMARCA4 antibody (83310-2-RR, Clone: 240172F4 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83310-2-PBS in a different storage buffer formulation.

• 

  FC experiment of Jurkat using 83310-2-RR (same clone as 83310-2-PBS)

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug SMARCA4 Recombinant antibody (83310-2-RR, Clone:240172F4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83310-2-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP00334-1

  Cytometric bead array standard curve of MP00334-1, SMARCA4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83310-1-PBS. Detection antibody: 83310-2-PBS. Standard: Ag16256. Range: 0.313-40 ng/mL.

• 

  Affinity and Kinetic Characterization of 83310-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83310-2-RR against Human SMARCA4 were performed. The affinity constant is 3.66 nM.

---

Product Information

83310-2-PBS targets SMARCA4 as part of a matched antibody pair:

MP00334-1: 83310-1-PBS capture and 83310-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SMARCA4 fusion protein Ag16256
• Full Name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4
• Calculated Molecular Weight: 1647 aa, 185 kDa
• GenBank Accession Number: BC150298
• Gene Symbol: SMARCA4
• Gene ID (NCBI): 6597
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.