Various lysates were subjected to SDS PAGE followed by western blot with 83310-2-RR (SMARCA4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 83310-2-RR (SMARCA4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IHC staining of human placenta using 83310-2-RR
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83310-2-RR (SMARCA4 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83310-2-RR (SMARCA4 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 83310-2-RR
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83310-2-RR (SMARCA4 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83310-2-RR (SMARCA4 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse cerebellum using 83310-2-RR
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 83310-2-RR (SMARCA4 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 83310-2-RR (SMARCA4 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat cerebellum using 83310-2-RR
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 83310-2-RR (SMARCA4 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 83310-2-RR (SMARCA4 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat cerebellum using 83310-2-RR
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 83310-2-RR (SMARCA4 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 83310-2-RR (SMARCA4 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat colon using 83310-2-RR
Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 83310-2-RR (SMARCA4 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 83310-2-RR (SMARCA4 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat colon using 83310-2-RR
Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 83310-2-RR (SMARCA4 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 83310-2-RR (SMARCA4 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat lung using 83310-2-RR
Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 83310-2-RR (SMARCA4 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 83310-2-RR (SMARCA4 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat lung using 83310-2-RR
Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 83310-2-RR (SMARCA4 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 83310-2-RR (SMARCA4 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse colon using 83310-2-RR
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 83310-2-RR (SMARCA4 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with .
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 83310-2-RR (SMARCA4 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with .
IHC staining of mouse colon using 83310-2-RR
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 83310-2-RR (SMARCA4 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with .
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 83310-2-RR (SMARCA4 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with .
IF Staining of HepG2 using 83310-2-RR
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SMARCA4 antibody (83310-2-RR, Clone: 240172F4 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SMARCA4 antibody (83310-2-RR, Clone: 240172F4 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
FC experiment of Jurkat using 83310-2-RR
1x10^6 Jurkat cells were intracellularly stained with 0.25 ug SMARCA4 Recombinant antibody (83310-2-RR, Clone:240172F4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
1x10^6 Jurkat cells were intracellularly stained with 0.25 ug SMARCA4 Recombinant antibody (83310-2-RR, Clone:240172F4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
Affinity and Kinetic Characterization of 83310-2-RR
Biolayer interferometry (BLl) kinetic assays of 83310-2-RR against Human SMARCA4 were performed. The affinity constant is 3.66 nM.
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
human placenta tissue, mouse cerebellum tissue, mouse colon tissue, mouse lung tissue, rat cerebellum tissue, rat colon tissue, rat lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HepG2 cells
Positive FC (Intra) detected in
Jurkat cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:5000-1:50000
Immunohistochemistry (IHC)
IHC : 1:2000-1:8000
Immunofluorescence (IF)/ICC
IF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)
FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
83310-2-RR targets SMARCA4 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
SMARCA4, also named as BAF190A, BRG1, SNF2B and SNF2L4, belongs to the SNF2/RAD54 helicase family. SMARCA4 is a transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. It is a component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. It is also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.
Various lysates were subjected to SDS PAGE followed by western blot with 83310-2-RR (SMARCA4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human placenta using 83310-2-RR
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83310-2-RR (SMARCA4 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 83310-2-RR
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83310-2-RR (SMARCA4 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse cerebellum using 83310-2-RR
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 83310-2-RR (SMARCA4 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse cerebellum using 83310-2-RR
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 83310-2-RR (SMARCA4 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse lung using 83310-2-RR
Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 83310-2-RR (SMARCA4 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse lung using 83310-2-RR
Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 83310-2-RR (SMARCA4 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat cerebellum using 83310-2-RR
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 83310-2-RR (SMARCA4 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat cerebellum using 83310-2-RR
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 83310-2-RR (SMARCA4 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat colon using 83310-2-RR
Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 83310-2-RR (SMARCA4 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat colon using 83310-2-RR
Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 83310-2-RR (SMARCA4 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat lung using 83310-2-RR
Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 83310-2-RR (SMARCA4 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat lung using 83310-2-RR
Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 83310-2-RR (SMARCA4 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse colon using 83310-2-RR
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 83310-2-RR (SMARCA4 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with .
IHC staining of mouse colon using 83310-2-RR
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 83310-2-RR (SMARCA4 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with .
IF/ICC Figures
IF Staining of HepG2 using 83310-2-RR
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SMARCA4 antibody (83310-2-RR, Clone: 240172F4 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
FC (INTRA) Figures
FC experiment of Jurkat using 83310-2-RR
1x10^6 Jurkat cells were intracellularly stained with 0.25 ug SMARCA4 Recombinant antibody (83310-2-RR, Clone:240172F4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
AFFINITY Figures
Affinity and Kinetic Characterization of 83310-2-RR
Biolayer interferometry (BLl) kinetic assays of 83310-2-RR against Human SMARCA4 were performed. The affinity constant is 3.66 nM.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
SMARCA4 Recombinant antibody
Catalog Number
83310-2-RR
Citations
-
Dilutions
WB : 1:5000-1:50000 IHC : 1:2000-1:8000 IF/ICC : 1:125-1:500 FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
Applications
WB, IHC, IF/ICC, FC (Intra), ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
