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SMARCA4 Recombinant antibody

SMARCA4 Recombinant Antibody for WB, ELISA, IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

Human, rat, mouse

Applications

WB, ELISA, IF/ICC

Conjugate

Unconjugated

CloneNo.

240172F4

Cat no : 83310-2-RR

Synonyms

BRG1, BAF190A, BAF190, ATP-dependent helicase SMARCA4, ATP dependent helicase SMARCA4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SMARCA4 Recombinant antibody (83310-2-RR)

    WB analysis using 83310-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83310-2-RR (SMARCA4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SMARCA4 Recombinant antibody (83310-2-RR)

    IF Staining of HepG2 using 83310-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SMARCA4 antibody (83310-2-RR, Clone: 240172F4 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, HSC-T6 cells, NIH/3T3 cells, HEK-293T cells, C6 cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83310-2-RR is SMARCA4 Fusion Protein expressed in E. coli.

Tested Reactivity Human, rat, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SMARCA4 fusion protein Ag16256
Full Name SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4
Calculated Molecular Weight 1647 aa, 185 kDa
Observed Molecular Weight 185 kDa
GenBank Accession NumberBC150298
Gene Symbol SMARCA4
Gene ID (NCBI) 6597
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SMARCA4, also named as BAF190A, BRG1, SNF2B and SNF2L4, belongs to the SNF2/RAD54 helicase family. SMARCA4 is a transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. It is a component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. It is also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.

Protocols

Product Specific Protocols
WB protocol for SMARCA4 antibody 83310-2-RRDownload protocol
IF protocol for SMARCA4 antibody 83310-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols