CoraLite® Plus 750-conjugated SLC25A5 Recombinant antibody

SLC25A5 Uni-rAb^TM Recombinant Antibody for WB

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB

Conjugate

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

230247B1

Cat no : CL750-83104

Synonyms

ANT1/2, ADP/ATP translocase 2, N-terminally processed, ADP/ATP translocase 2, ADP,ATP carrier protein 2, AAC2

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Validation Data Gallery

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  WB analysis using CL750-83104

  Various lysates were subjected to SDS PAGE followed by western blot with CL750-83104 (SLC25A5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  FC experiment of PC-3 using CL750-83104

  1x10^6 PC-3 cells were intracellularly stained with 0.4 ug CoraLite® Plus 750-conjugated SLC25A5 Recombinant antibody (CL750-83104, Clone:230247B1)(red), or 0.4 ug Isotype Control (CL750-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: C6 cells, rat liver tissue, mouse liver tissue, Neuro-2a cells
• Positive FC (Intra) detected in: PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL750-83104 targets SLC25A5 in WB applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SLC25A5 fusion protein Ag11956
• Full Name: solute carrier family 25 (mitochondrial carrier; adenine nucleotide translocator), member 5
• Calculated Molecular Weight: 298 aa, 33 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC056160
• Gene Symbol: SLC25A5
• Gene ID (NCBI): 292
• Conjugate: CoraLite® Plus 750 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 755 nm / 780 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Adenine nucleotide translocators (ANTs) are mitochondrial carrier proteins that exchange ATP and ADP between the cytoplasm and the mitochondrial matrix. Four isoforms of ANT were identified: ANT1 (encoded by SLC25A4) is highly expressed in different tissues; ANT2 (encoded by SLC25A5) is predominantly expressed in proliferating, regenerating, or undifferentiated cells; ANT3 is ubiquitously expressed; ANT4 is mainly expressed in testis and germ cells. This antibody recognizes both of ANT1 and ANT2. The predicted molecular weight of ANT1/2 protein is around 32 kDa. While 60 kDa protein can also be observed, which has been proposed as the dimer of ANT1/2. (22423112)