ANT1/2 Recombinant antibody

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  WB analysis using 83104-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83104-1-RR (SLC25A5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

ANT1/2 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 83104-1-RR

Clone No.230247B1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

SLC25A5, ADP/ATP translocase 2, N-terminally processed, ADP/ATP translocase 2, ADP,ATP carrier protein 2, AAC2

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Tested Applications

• Positive WB detected in: HepG2 cells, C6 cells, NIH/3T3 cells, COS-7 cells, Neuro-2a cells, rat liver tissue, mouse liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83104-1-RR targets ANT1/2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ANT1/2 fusion protein Ag11956
• Full Name: solute carrier family 25 (mitochondrial carrier; adenine nucleotide translocator), member 5
• Calculated Molecular Weight: 298 aa, 33 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC056160
• Gene Symbol: SLC25A5
• Gene ID (NCBI): 292
• RRID: AB_3670817
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P05141
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Adenine nucleotide translocators (ANTs) are mitochondrial carrier proteins that exchange ATP and ADP between the cytoplasm and the mitochondrial matrix. Four isoforms of ANT were identified: ANT1 (encoded by SLC25A4) is highly expressed in different tissues; ANT2 (encoded by SLC25A5) is predominantly expressed in proliferating, regenerating, or undifferentiated cells; ANT3 is ubiquitously expressed; ANT4 is mainly expressed in testis and germ cells. This antibody recognizes both of ANT1 and ANT2. The predicted molecular weight of ANT1/2 protein is around 32 kDa. While 60 kDa protein can also be observed, which has been proposed as the dimer of ANT1/2. (22423112)