Anti-Human SLAM/CD150 Rabbit Recombinant Antibody

SLAM/CD150 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

241800B12

Cat no : 98220-1-RR

Synonyms

SLAMF1, SLAM family member 1, SLAM, IPO-3, IPO 3

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Validation Data Gallery

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  FC experiment of human PBMCs using 98220-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human SLAM/CD150 Rabbit Recombinant Antibody (98220-1-RR, Clone: 241800B12) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 98220-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human SLAM/CD150 Rabbit Recombinant Antibody (98220-1-RR, Clone: 241800B12) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. Lymphocytes were gated.

• 

  Affinity and Kinetic Characterization of 98220-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98220-1-RR against Human SLAM/CD150 were performed. The affinity constant is 3.16 nM.

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98220-1-RR targets SLAM/CD150 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: signaling lymphocytic activation molecule family member 1
• Calculated Molecular Weight: 37kDa
• GenBank Accession Number: NM_003037.5
• Gene Symbol: CD150
• Gene ID (NCBI): 6504
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

Signaling lymphocytic activation molecule (SLAM, also known as SLAMF1 or CD150) is a 70-95 kDa transmembrane glycoprotein that belongs to the immunoglobulin gene superfamily. SLAM is constitutively expressed on peripheral blood memory T-cells, T-cell clones, immature thymocytes and a proportion of B-cells, and is rapidly induced on naive T-cells after activation. SLAM is involved in T cell stimulation and cytokine production, and may play an important role in the regulation of immune responses to pathogens.