CoraLite® Plus 750-conjugated SECTM1 Monoclonal antibody

SECTM1 Monoclonal Antibody for WB

Host / Isotype

Mouse / IgM

Reactivity

human

Applications

WB

Conjugate

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

1C11B4

Cat no : CL750-60281

Synonyms

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Validation Data Gallery

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  WB analysis using CL750-60281

  Various lysates were subjected to SDS PAGE followed by western blot with CL750-60281 (SECTM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: K-562 cells

Mouse monoclonal antibodies of IgM isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL750-60281 targets SECTM1 in WB applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgM
• Class: Monoclonal
• Type: Antibody
• Immunogen: SECTM1 fusion protein Ag14645
• Full Name: secreted and transmembrane 1
• Calculated Molecular Weight: 248 aa, 27 kDa
• Observed Molecular Weight: 27 kDa
• GenBank Accession Number: BC017716
• Gene Symbol: SECTM1
• Gene ID (NCBI): 6398
• Conjugate: CoraLite® Plus 750 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 755 nm / 780 nm
• Form: Liquid
• Purification Method: Thiophilic affinity chromatograph
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

SECTM1, also named as protein K12, is originally identified by its location just upstream of the CD7 locus. It has been proposed as a ligand for CD7. SECTM1 is involved in thymocyte signaling. This antibody is specific to SECTM1.