SAE1 Recombinant antibody

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  IHC staining of human colon cancer using 84516-6-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84516-6-RR (SAE1 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 84516-6-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84516-6-RR (SAE1 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 84516-6-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SAE1 antibody (84516-6-RR, Clone: 241923F12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of MCF-7 using 84516-6-RR

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using SAE1 antibody (84516-6-RR, Clone: 241923F12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of A549 using 84516-6-RR

  1x10^6 A549 cells were intracellularly stained with 0.25 ug SAE1 Recombinant antibody (84516-6-RR, Clone:241923F12) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  Affinity and Kinetic Characterization of 84516-6-RR

  Biolayer interferometry (BLl) kinetic assays of 84516-6-RR against Human SAE1 were performed. The affinity constant is 1.52 nM.

SAE1 {ptg:Brand} Recombinant Antibody for IHC, IF/ICC, FC (Intra), ELISA

Cat No. 84516-6-RR

Clone No.241923F12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, FC (Intra), ELISA

Synonyms

241923F12, AOS1, HSPC140, Sentrin/SUMO activating protein AOS1, SUA1

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Tested Applications

• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells, HeLa cells
• Positive FC (Intra) detected in: A549 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:300-1:1200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84516-6-RR targets SAE1 in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SAE1 fusion protein Ag0275
• Full Name: SUMO1 activating enzyme subunit 1
• Calculated Molecular Weight: 38 kDa
• GenBank Accession Number: BC000344
• Gene Symbol: SAE1
• Gene ID (NCBI): 10055
• RRID: AB_3672022
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q9UBE0
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SAE1(SUMO-activating enzyme subunit 1) is also named as AOS1, SUA1, UBLE1A and belongs to the ubiquitin-activating E1 family. SAE1 has a calculated molecular mass of 38 kD. SAE1 and UBA2 form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins(PMID:9920803). Western blot analysis of synchronized HeLa cells detectes increased AOS1 expression as cells progressed through S phase, followed by a substantial decrease in G2 phase. Immunofluorescence analysis shows AOS1 and UBA2 distributed throughout nuclei, but they are excluded from nucleoli.(PMID:11481243).