RPP30 Recombinant antibody

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  WB analysis using 83481-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83481-5-RR (RPP30 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary cancer using 83481-5-RR

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83481-5-RR (RPP30 antibody) at dilution of 1:1500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 83481-5-RR

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83481-5-RR (RPP30 antibody) at dilution of 1:1500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U-251 using 83481-5-RR

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using RPP30 antibody (83481-5-RR, Clone: 240468E4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of U-251 using 83481-5-RR

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using RPP30 antibody (83481-5-RR, Clone: 240468E4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of U-251 using 83481-5-RR

  1x10^6 U-251 cells were intracellularly stained with 0.25 ug RPP30 Recombinant antibody (83481-5-RR, Clone:240468E4) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  Affinity and Kinetic Characterization of 83481-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83481-5-RR against Human RPP30 were performed. The affinity constant is below 1 pM.

RPP30 Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Cat No. 83481-5-RR

Clone No.240468E4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

TSG15, RNASEP2, RNaseP protein p30, RNase P subunit 2, 240468E4

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, NIH/3T3 cells, U-251 cells
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U-251 cells
• Positive FC (Intra) detected in: U-251 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:750-1:3000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83481-5-RR targets RPP30 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: RPP30 fusion protein Ag8600
• Full Name: ribonuclease P/MRP 30kDa subunit
• Calculated Molecular Weight: 268 aa, 29 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC006991
• Gene Symbol: RPP30
• Gene ID (NCBI): 10556
• RRID: AB_3671109
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P78346
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RPP30 is a part of multimeric ribonuclease P complex and ribonuclease MRP complex. It is also a component of the MRP ribonuclease complex, which cleaves pre-rRNA sequences.