RPP30 Recombinant antibody, PBS Only (Detector)

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  WB analysis using 83481-5-RR (same clone as 83481-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83481-5-RR (RPP30 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83481-5-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP00472-1

  Sandwich ELISA standard curve of MP00472-1, Human RPP30 Recombinant Matched Antibody Pair - PBS only. 83481-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag8600. 83481-5-PBS was HRP conjugated as the detection antibody. Range: 3.13-200 ng/mL

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  Sample test of MP00472-1

  HEK-293 and U-251 cell lysate were measured. The human RPP30 concentration of detected samples was determined to be 194.20 ng/mL (based on a 1.2 mg/mL extract load) in HEK-293 cell lysate and 139.41 ng/mL (based on a 3.3 mg/mL extract load) in U-251 cell lysate.

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  IHC staining of human ovary cancer using 83481-5-RR (same clone as 83481-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83481-5-RR (RPP30 antibody) at dilution of 1:1500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83481-5-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 83481-5-RR (same clone as 83481-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83481-5-RR (RPP30 antibody) at dilution of 1:1500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83481-5-PBS in a different storage buffer formulation.

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  IF Staining of U-251 using 83481-5-RR (same clone as 83481-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using RPP30 antibody (83481-5-RR, Clone: 240468E4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83481-5-PBS in a different storage buffer formulation.

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  IF Staining of U-251 using 83481-5-RR (same clone as 83481-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using RPP30 antibody (83481-5-RR, Clone: 240468E4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83481-5-PBS in a different storage buffer formulation.

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  FC experiment of U-251 using 83481-5-RR (same clone as 83481-5-PBS)

  1x10^6 U-251 cells were intracellularly stained with 0.25 ug RPP30 Recombinant antibody (83481-5-RR, Clone:240468E4) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83481-5-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00472-1

  Cytometric bead array standard curve of MP00472-1, RPP30 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83481-2-PBS. Detection antibody: 83481-5-PBS. Standard: Ag8600. Range: 0.78-100 ng/mL.

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  Affinity and Kinetic Characterization of 83481-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83481-5-RR against Human RPP30 were performed. The affinity constant is below 1 pM.

RPP30 {ptg:Brand} Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 83481-5-PBS

Clone No.240468E4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

TSG15, RNASEP2, RNaseP protein p30, RNase P subunit 2, 240468E4

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Product Information

83481-5-PBS targets RPP30 as part of a matched antibody pair:

MP00472-1: 83481-2-PBS capture and 83481-5-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: RPP30 fusion protein Ag8600
• Full Name: ribonuclease P/MRP 30kDa subunit
• Calculated Molecular Weight: 268 aa, 29 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC006991
• Gene Symbol: RPP30
• Gene ID (NCBI): 10556
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P78346
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

RPP30 is a part of multimeric ribonuclease P complex and ribonuclease MRP complex. It is also a component of the MRP ribonuclease complex, which cleaves pre-rRNA sequences.