CoraLite® Plus 488-conjugated RORA Recombinant antibody

RORA Recombinant Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

230217G6

Cat no : CL488-82930

Synonyms

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Validation Data Gallery

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  IF Staining of HepG2 using CL488-82930

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 RORA antibody (CL488-82930, Clone: 230217G6 ) at dilution of 1:200.

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-82930 targets RORA in IF/ICC applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: RORA fusion protein Ag0944
• Full Name: RAR-related orphan receptor A
• Calculated Molecular Weight: 59 kDa
• Observed Molecular Weight: 60-66 kDa
• GenBank Accession Number: BC008831
• Gene Symbol: RORA
• Gene ID (NCBI): 6095
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

RORA is a steroid hormone receptor and has been implicated in the pathology of circadian rhythms, bone growth, angiogenesis, development of cones, cellular metabolism and a mediator in the immune and lipid metabolism pathways [PMID:21998696]. It binds DNA as a monomer to hormone response elements (HRE) containing a single core motif half-site preceded by a short A-T-rich sequence, which binds to the consensus sequence 5'-[AT][TA]A[AT][CGT]TAGGTCA-3'. Regulates a number of genes involved in lipid metabolism such as apolipoproteins AI, APOA5, CIII, CYP71 and PPAR gamma, in cerebellum and photoreceptor development including PCP2, OPN1SW, OPN1SM and ARR3, in circadian rhythm with BMAL1, and skeletal muscle development with MYOD1 [PMID:11053433,14570920].