RORA Recombinant antibody

RORA Recombinant Antibody for WB, IF, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Rat

Applications

WB, IF, FC, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

230217G6

Cat no : 82930-1-RR

Synonyms

DKFZp686M2414, NR1F1, Nuclear receptor ROR alpha, Nuclear receptor RZR alpha, RAR related orphan receptor A, ROR1, ROR2, ROR3, RORA, RZR ALPHA, RZRA



Tested Applications

Positive WB detected inA549 cells, HepG2 cells, L02 cells, HuH-7 cells, PC-3 cells
Positive IHC detected inrat cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:150-1:600
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82930-1-RR targets RORA in WB, IF, FC, IHC, ELISA applications and shows reactivity with Human, Rat samples.

Tested Reactivity Human, Rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RORA fusion protein Ag0944
Full Name RAR-related orphan receptor A
Calculated Molecular Weight 59 kDa
Observed Molecular Weight60-66 kDa
GenBank Accession NumberBC008831
Gene Symbol RORA
Gene ID (NCBI) 6095
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RORA is a steroid hormone receptor and has been implicated in the pathology of circadian rhythms, bone growth, angiogenesis, development of cones, cellular metabolism and a mediator in the immune and lipid metabolism pathways [PMID:21998696]. It binds DNA as a monomer to hormone response elements (HRE) containing a single core motif half-site preceded by a short A-T-rich sequence, which binds to the consensus sequence 5'-[AT][TA]A[AT][CGT]TAGGTCA-3'. Regulates a number of genes involved in lipid metabolism such as apolipoproteins AI, APOA5, CIII, CYP71 and PPAR gamma, in cerebellum and photoreceptor development including PCP2, OPN1SW, OPN1SM and ARR3, in circadian rhythm with BMAL1, and skeletal muscle development with MYOD1 [PMID:11053433,14570920].

Protocols

Product Specific Protocols
WB protocol for RORA antibody 82930-1-RRDownload protocol
IHC protocol for RORA antibody 82930-1-RRDownload protocol
IF protocol for RORA antibody 82930-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols