MIRO1 Recombinant antibody, PBS Only

MIRO1 Uni-rAb^TM Recombinant Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241157D1

Cat no : 84055-5-PBS

Synonyms

RHOT1, Rac-GTP-binding protein-like protein, MIRO 1, hMiro 1, EC:3.6.5.-

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Validation Data Gallery

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  WB analysis using 84055-5-RR (same clone as 84055-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84055-5-RR (MIRO1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84055-5-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 84055-5-RR (same clone as 84055-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 84055-5-RR (MIRO1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84055-5-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 84055-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83945-5-RR against Human RHOT1 were performed. The affinity constant is below 1 pM.

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Product Information

The immunogen of 84055-5-PBS is MIRO1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: MIRO1 fusion protein Ag15605
• Full Name: ras homolog gene family, member T1
• Calculated Molecular Weight: 618 aa, 71 kDa
• Observed Molecular Weight: 75 kDa
• GenBank Accession Number: BC060781
• Gene Symbol: MIRO1
• Gene ID (NCBI): 55288
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.