MIRO1 Recombinant antibody

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  WB analysis using 84055-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84055-5-RR (MIRO1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 84055-5-RR

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 84055-5-RR (MIRO1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 84055-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83945-5-RR against Human RHOT1 were performed. The affinity constant is below 1 pM.

MIRO1 Uni-rAb^TM Recombinant Antibody for WB, IHC, ELISA

Cat No. 84055-5-RR

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

RHOT1, Rac-GTP-binding protein-like protein, MIRO 1, hMiro 1, EC:3.6.5.-

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, HL-60 cells, mouse testis tissue, mouse heart tissue, mouse brain tissue, rat brain tissue
• Positive IHC detected in: mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84055-5-RR targets MIRO1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: MIRO1 fusion protein Ag15605
• Full Name: ras homolog gene family, member T1
• Calculated Molecular Weight: 618 aa, 71 kDa
• Observed Molecular Weight: 75 kDa
• GenBank Accession Number: BC060781
• Gene Symbol: MIRO1
• Gene ID (NCBI): 55288
• RRID: AB_3671623
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q8IXI2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.