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MIRO1 Recombinant antibody

MIRO1 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

241157D1

Cat no : 84055-5-RR

Synonyms

RHOT1, Rac-GTP-binding protein-like protein, MIRO 1, hMiro 1, EC:3.6.5.-

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MIRO1 Recombinant antibody (84055-5-RR)

    WB analysis using 84055-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84055-5-RR (MIRO1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using MIRO1 Recombinant antibody (84055-5-RR)

    IHC staining of mouse kidney using 84055-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 84055-5-RR (MIRO1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84055-5-RR

    Affinity and Kinetic Characterization of 84055-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83945-5-RR against Human RHOT1 were performed. The affinity constant is below 1 pM.

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HL-60 cells, mouse testis tissue, mouse heart tissue, mouse brain tissue, rat brain tissue
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84055-5-RR is MIRO1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MIRO1 fusion protein Ag15605
Full Name ras homolog gene family, member T1
Calculated Molecular Weight 618 aa, 71 kDa
Observed Molecular Weight75 kDa
GenBank Accession NumberBC060781
Gene Symbol MIRO1
Gene ID (NCBI) 55288
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for MIRO1 antibody 84055-5-RRDownload protocol
IHC protocol for MIRO1 antibody 84055-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols