RBM17 Recombinant antibody, PBS Only

RBM17 Uni-rAb^TM Recombinant Antibody for IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230125F6

Cat no : 82889-1-PBS

Synonyms

SPF45, SPF 45, RNA-binding motif protein 17, RBM 17, 45 kDa-splicing factor

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Validation Data Gallery

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  IP experiment of MCF-7 using 82889-1-RR (same clone as 82889-1-PBS)

  IP result of anti-RBM17 (IP:82889-1-RR, 4ug; Detection:82889-1-RR 1:10000) with MCF-7 cells lysate 1470 ug. This data was developed using the same antibody clone with 82889-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 82889-1-RR (same clone as 82889-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RBM17 antibody (82889-1-RR, Clone: 230125F6 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004).(CL488-Phalloidin, green). This data was developed using the same antibody clone with 82889-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 82889-1-RR (same clone as 82889-1-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug RBM17 Recombinant antibody (82889-1-RR, Clone:230125F6) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 82889-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82889-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82889-1-RR against Human RBM17 were performed. The affinity constant is 1.54 nM.

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Product Information

82889-1-PBS targets RBM17 in IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: RBM17 fusion protein Ag0985
• Full Name: RNA binding motif protein 17
• Calculated Molecular Weight: 45 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: BC007871
• Gene Symbol: RBM17
• Gene ID (NCBI): 84991
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

RNA-binding motif protein 17 (RBM17), also named splicing factor 45, was first identified as a member of the spliceosome. RBM17 expression is low in normal tissues but is overexpressed in several forms of cancer, including breast, ovarian and prostate.